Decreas of BCL-2 Expression by Ethanol Extract of Ocinum basilicum L. Leaves in Breast Cancer Cells

Authors

  • Devi Nisa Hidayati Fakultas Farmasi, Universitas Wahid Hasyim
  • Fatimatuz Zahroh Fakultas Farmasi, Universitas Wahid Hasyim
  • Lina Wahyuni Fakultas Farmasi, Universitas Wahid Hasyim
  • Ibrahim Arifin Fakultas Farmasi, Universitas Wahid Hasyim

DOI:

https://doi.org/10.33533/jpm.v15i1.2491

Keywords:

Ocinum basilicum L, BCL-2, T47D, MCF-7

Abstract

Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.

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Published

2021-03-14

How to Cite

Hidayati, D. N., Zahroh, F., Wahyuni, L., & Arifin, I. (2021). Decreas of BCL-2 Expression by Ethanol Extract of Ocinum basilicum L. Leaves in Breast Cancer Cells. Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 15(1). https://doi.org/10.33533/jpm.v15i1.2491

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Vol. 15 No. 1 (2021): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan

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Article

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