UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP ISOLAT BAKTERI Escherichia coli JAJANAN CILOK SECARA IN VITRO DENGAN METODE DIFUSI

Fadel Abima, Meiskha Bahar, Aulia Chairani

Sari


Diarrhea is still one of the world’s major health problem especially in developing country. Foods and beverages contaminated by microorganism become the risk factor of diarrhea, including Escherichia coli (E. coli). One of the curative effort that can be done is to utilise the secondary metabolite compounds contained in binahong leaf (Anredera cordifolia (Ten.) Steenis). This study used true experimental method. The sample was binahong leaf extract with concentration of 25%, 50, and 75% using diffusion method (Kirby-Bauer) on doublelayered Mueller Hinton Agar (MHA) (base layer and seed layer) in order to measure the growth inhibition zone around the cylinder plate. The amount of population on this study was 24 isolations of E. coli. The repetition of each treatment group was counted by Federer formula. The result analyzed using Kruskal-Wallis test and showed that there was difference on binahong leaf extract effectiveness (p < 0,05), as well as on post hoc analysis using MannWhitney test showed that there was significant difference at concentration of 25%,50%, and 75%. (p < 0,001). This shows that there is a significant differences in the concentration of binahong leaf extract.

Teks Lengkap:

PDF

Referensi


Adisasmito W. Faktor risiko diare pada bayi dan balita di Indonesia; systematic review penelitian akademik bidang kesehatan masyarakat. Makara. 2007;11(1)

World Health Organization 2013, Diarrhoeal disease; 2013. Available from : http://www.who.int/mediacentre/factsheets/fs330/en/ (AccessedApril 9, 2016).

Kementerian Kesehatan RI, Situasi diare di Indonesia, Jakarta : Kementerian Kesehatan RI; 2011.

Sari M. Uji bakteriologis dan resistensi antibiotik terhadap bakteri Escherichia coli dan Shigella sp pada makanan gado-gado di kantin UIN Syarif Hidayatullah Jakarta.Available from http://repository.uinjkt.ac.id/dspace/bits tream/123456789/29523/1/Mulia%20Sari-fkik.pdf[AccessedApril9, 2015].

Djaja IM. Kontaminasi Eschericia. Coli pada makanan dari tiga jenis tempat pengelolaan makanan (TPM) di Jakarta Selatan 2003. Makara. 2008; 12(1)

Jawetz, Melnick, & Adelberg. In Brooks GF, Butel JS, Morse SA, (editors). Mikrobiologi Kedokteran, Jakarta, EGC; 2008.

Noviana H.Pola kepekaan antibiotika Escherichia coli yang diisolasi dari berbagaispesimen klinis, Jurnal Kedokteran Trisakti. 2004, 23(4).

Martha D, Achmad S, Tejasari, Escherichia coli resisten terhadap seftriaksondan siprofloksasin : Prosiding Pendidikan Dokter Universitas Islam Bandung, Bandung; 2015

Rochani N. Uji aktivitas antijamur ekstrak daun binahong (Anrederacordifolia (Tenore) Steen) terhadap Candida albicans serta skrining fitokimianya; 2009. Available from http://eprints.ums.ac.id/5267/1/K100050305.pdf [Accessed Desember 25,2015].

Araruna, MKA, Santos KKA, da Costa JGM, Coutinho, HDM, Boligon, AA, Stefanello ST, Athayde ML, Saraiva RA, da Rocha JBT, Kerntopf, MR, de Menezes IRA.Phenolic composition and in vitro activity of the Brazilian fruit tree CaryocarcoriaceumWittm; 2012. Eur J of Integrative Medicine. 2013.5(2) :178-183. Available from https://www.sciencedirect.com/science/article/pii/S1876382012011250. [Accessed Maret 26, 2015].

Emilia, I. Isolasi dan identifikasi senyawa alkaloid dari daun tumbuhan sengugu (Clerodendron serratum Spreng), Sainmatika. 2010,7(2).

Soeksmanto A. Pengaruh ekstrak butanol buah tua mahkota dewa (Phaleriamacrocarpa) terhadap jaringan ginjal mencit (Mus musculus), Biodiversitas.2006; 7(3).

Rosyidah K, Nurmuhaimina SA, Komari N, Astuti MD. Aktivitas antibakteri fraksi saponin dari kulit batang tumbuhan kasturi (Mangifera casturi). Journal of Chemistry. 2006. 1(2).Available from http://ejournal.uinmalang.ac.id/index.php/Kimia/article/view/1674 [Accessed December 2, 2015].




DOI: http://dx.doi.org/10.33533/jpm.v11i1.205

Article Metrics

Sari view : 1099 times
PDF - 208 times

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

 


width="113"

Gedung Wahidin Sudirohusodo, UPN Veteran Jakarta                     

Jl. RS Fatmawati No 1, Pondok Labu, Jakarta Selatan

Phone: 021-7656904 ; Fax: 021-7692860