Abstract
ABSTRACT
Hand sanitizer is a hand cleanser which is easy and practical in use. Using hand sanitizer can be an alternative for washing hands if in condition without water and soap. Hand sanitizer is used to clean pathogens on hands. The ability to kill pathogens in hand sanitizers is due to the active substance content in them. In this research, the effectiveness of 2 hand sanitizers on the market was tested. The test aims to see the effect of the active substances in each preparation on the effectiveness of killing pathogens. Testing was carried out using the Kirby Bauer method and on 2 bacterial media, namely Escherichia coli and Shigella sonnei. Hand sanitizer 1 has a composition consisting of 70% ethyl alcohol and 0.5% chlorhexidine. Hand sanitizer 2 has a composition consisting of 55% ethyl alcohol and 18% isopropyl alcohol with a total of 73% alcohol. Based on observations, it was found that hand sanitizer 1 was more effective than hand sanitizer 2. The chlorhexidine content in hand sanitizer 1 increased its effectiveness in killing pathogens.
References
Aini, F. (2018). Isolasi dan identifikasi Shigella sp. penyebab diare pada balita. Bio-Site, 4(1), 1–40. https://doi.org/10.22437/bs.v4i1.5012
Al-Adham, I., Haddadin, R., Collier, P., Fraise, A. P., Maillard, J.Y., & Sattar, S.A. (Eds.). (2013). Types of microbicidal and microbistatic agents. Blackwell Publishing. https://doi.org/10.1002/9781118425831.ch2
Aljufri, A.Q. (2015). Perbedaan efektivitas antiseptik triclosan dengan handrub chlorhexidine gluconate etanol dalam menurunkan kuantitas bakteri telapak tangan [Undergraduate honors thesis, Universitas Islam Sultan Agung]. Fakultas Kedokteran UNISSULA. http://repository.unissula.ac.id/id/eprint/255
Amalia, S., Wahdaningsih, S., & Untari, E. K. (2014). Uji aktivitas fraksi n heksan kulit buah naga merah (Hylocereus polyrhizus Britton & Rose) terhadap bakteri Staphylococcus aureus atcc 25923. Jurnal Fitofarmaka Indonesia, 1(2), 61–64. https://doi.org/10.33096/jffi.v1i2.191
Ambari, Y., Ningsih, A. W., Sinaga, B., & Nurrosyidah, I. H. (2020). Efektivitas antiseptik gel hand sanitizer ekstrak etanol pelepah pisang kepok (Musa paradisiaca L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Ilmiah Medicamento, 6(2), 123–132. https://doi.org/10.36733/medicamento.v6i2.1107
Asngad, A., R. A. B., & Nopitasari. (2018). Kualitas gel pembersih tangan (hand sanitizer) dari ekstrak batang pisang dengan penambahan alkohol, triclosan dan gliserin yang berbeda dosisnya. Jurnal Bioeksperimen, 4(2), 61–70. https://doi.org/10.23917/bioeksperimen.v4i2.6888
Badjoeri, M. (2007). Bakteri Escherichia coli pada beberapa sumur penduduk dan sungai di wilayah pasar krui dan Desa Rawas, Lampung Barat. Jurnal Biologi Indonesia, 4(4), 249–253. https://doi.org/10.14203/jbi.v4i4.3252
Baizuroh, N., Yahdi, & Dewi, Y. K. (2021). Uji kualitas hand sanitizer ekstrak daun kunyit (Curcuma longa Linn). al Kimiya: Jurnal Ilmu Kimia dan Terapan, 8(1), 88–94. https://doi.org/10.15575/ak.v7i2.8744
Beloin, C., Roux, A., & Ghigo, J. M. (2008). Escherichia coli biofilms. Current Topics in Microbiology and Immunology, 322, 249–289. https://doi.org/10.1007/978-3-540-75418-3_12
Budiyanto, M. A. K. (2002). Mikrobiologi Terapan. Universitas Muhammadiyah Malang.
Elfidasari, D. (2011). Perbandingan kualitas es di lingkungan universitas al azhar indonesia dengan restoran fast food di daerah senayan dengan indikator jumlah Escherichia coli terlarut. Jurnal Al-Azhar Indonesia Seri Sains Dan Teknologi, 1(1), 18–23. http://doi.org/10.36722/sst.v1i1.14
Ellafi, A., Abdallah, F.B., Lagha, R., Harbi, B., & Bakhrouf, A. (2011). Biofilm production, adherence and morphological alterations of Shigella spp. under salt conditions. Annals of Microbiology, 61(4), 741–747. http://doi.org/10.1007/s13213-010-0190-5
Emelda, Safitri, E. A., & Fatmawati, A. (2021). Aktivitas inhibisi ekstrak etanolik Ulva lactuca terhadap bakteri Staphylococcus aureus. Pharmaceutical Journal of Indonesia, 7(1), 43–48. https://doi.org/10.21776/ub.pji.2021.007.01.7
Jawetz, E., Melnick, J. L., Adelberg, E. A., Brooks, G. F., Butel, J. S., & Ornston, L. N. (2007). Jawetz, Melnick & Adelberg’s Medical Microbiology (23th ed.) (Hartanto, H., Rachman, C., Dimanti, A., & Diani, A., Trans.). Penerbit Buku Kedokteran EGC.
Kementerian Kesehatan Republik Indonesia. (2012). Pedoman Bahan Berbahaya pada Produk Alat Kesehatan dan Perbekalan Kesehatan Rumah Tangga. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan Kementerian Kesehatan Republik Indonesia.
Karsinah, L. & Suharto, M. (2011). Buku ajar mikrobiologi kedokteran: Batang gram negatif Escherichia. Binarupa Aksara Publisher.
Kartikasari, A. M., Hamid, I. S., Purnama, M. T. E., Damayanti, R., Fikri, F., & Praja, R. N. (2019). Isolasi dan identifikasi bakteri Escherichia coli kontaminan pada daging ayam broiler di rumah potong ayam Kabupaten Lamongan. Jurnal Medik Veteriner, 2(1), 66–71. https://doi.org/10.20473/jmv.vol2.iss1.2019.66-71
Kuyyakanond, T. & Quesnel, L. B. (1992). The mechanism of action of chlorhexidine. FEMS Microbiology Letters, 100(1–3), 211–215. https://doi.org/10.1111/j.1574-6968.1992.tb14042.x
Komang, J.P.P., Agus H. (2019). Efek sinergis kombinasi chlorhexidine dan alkohol terhadap daya hambat pertumbuhan Staphylococcus aureus. e - Jurnal Medika Udayana, 8(3).
Lucchini, S., Liu, H., Jin, Q., Hinton, J. C., & Yu, J. (2005). Transcriptional adaptation of Shigella flexneri during infection of macrophages and epithelial cells: insights into the strategies of a cytosolic bacterial pathogen. Infection and Immunity, 73(1), 88–102. https://doi.org/10.1128/IAI.73.1.88-102.2005
Lusiana R. A., D. S. Widodo, L. Suyanti, Gunawan, A. Haris. (2020). Edukasi pembuatan hand sanitizer berbasis lidah buaya pada masyarakat Desa Harjowinangun, Grobogan. Jurnal Pengabdian Kepada Masyarakat (JPKM), Universitas Lampung, 1 (1).
https://doi.org/10.23960/jpkmt.v1i1.19
Mcdonnell, G., & Russell, A.D. (1999). Antiseptics and disinfectants: activity, action, and resistance. American society of microbiology. 12(1).
https://doi.org/10.1128/cmr.12.1.147
Nelintong N, Isnaeni, N. E. Nasution. (2015). Aktivitas antibakteri susu probiotik lactobacillus terhadap bakteri penyebab diare (Escherichia coli, Salmonella typhimurium, Vibrio cholerae). Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, 2 (1).
Nuryani, Siti., Putro, R. Fx. Saptono., Darwani. (2017). Pemanfaatan ekstrak daun jambu biji (Psidium guajava Linn) sebagai antibakteri dan antifungi. Jurnal Teknologi Laboratorium, 6(2),41-45.
http://dx.doi.org/10.29238/teknolabjournal.v6i2.95
Octaviani, M., Fadhli, H., & Yuneistya, E. 2019. Antimicrobial activity of ethanol extract of shallot (Allium cepa L.) peels. Pharmaceutical Sciences and Research. 6(1),62-68
https://doi.org/10.7454/psr.v6i1.4333
Osland, A.M., Vestby, L.K., & Nesse, L.L. (2020). The effect of disinfectants on quinolone resistant E. coli (QREC) in biofilm. Microorganism,2-11.
https://doi.org/10.3390/microorganisms8111831
Purwatiningsih, Sri. (2015). Skripsi. Pengaruh penggunaan hand sanitizer terhadap kepatuhan cuci tangan perawat pelaksana di ruang rawat inap RSU Assalam gemolong. stikes kusuma husada.
Putri, D. H., Fifendy, M., & Febrianti, R. (2016). Daya hambat sari tanaman obat terhadap pertumbuhan bakteri strain methicillin resistant Staphylococcus aureus (Mrsa). Sainstek: Jurnal Sains dan Teknologi. 2(2),125-129.
https://dx.doi.org/10.31958/js.v2i2.17
Ranjbar, R., & Farahani, A. (2019). Shigella: antibiotic-resistance mechanisms and new horizons for treatment. Infection and drug resistance, 3137-3167.
https://doi.org/10.2147/idr.s219755
Retnowati, Y., & Dama, L. (2009). Pembentukan biofilm oleh Escherichia coli dan resistensinya terhadap klorin. Jurnal Entropi. 4(1), 38-51.
Rini, E.P., Nugraheni, E.R. (2018). Uji daya hambat berbagai merek hand sanitizer gel terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Journal of Pharmaceutical Science and Clinical Research. 01.
https://doi.org/10.20961/jpscr.v3i1.15380
Setiawan A R, Salma N A, Nurhidayah G, Tukiran. (2022). Uji aktivitas antibakteri sediaan hand sanitizer berbahan etanol hasil evaporasi ekstrak kayu secang. Universitas Negeri Surabaya
Sinaga, E. M. (2017). Identifikasi bakteri Escherichia coli pada es kristal dengan menggunakan metode most probable number (MPN) yang diperjualbelikan oleh pedagang di Jalan Kapten Muslim Medan tahun 2017. Jurnal Mutiara Kesehatan Masyarakat. 10(7),41-47.
Sinaredi, B.R., Pradopo, S., Wibowo, T.B. (2014). Daya antibakteri obat kumur chlorhexidine, povidone iodine, fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis. Dental Journal Majalah Kedokteran Gigi. 47(4).
Situmeang, S. M. F. & Sembiring, T. J (2019). Efektivitas hand sanitizer dalam membunuh kuman di tangan. 1(1), 6-11.
Siswandono dan Soekardjo, B. (2016). Kimia Medisinal, 449-463, Airlangga University Press.
Srikartika, P., Suharti, N. & Anas, E. (2016). Kemampuan daya hambat bahan aktif beberapa merek dagang hand sanitizer terhadap pertumbuhan Staphylococcus Aureus. Jurnal Kesehatan Andalas. 5(3).
https://doi.org/10.25077/jka.v5i3.613
Sudarwati, D. (2016). Uji Aktivitas Senyawa Antibakteri Pada Ekstrak Daun Kelor dan Bunga Rosella. Indonesia Journal of Chemical Science, 5(1),1-4.
Susanty, T .Y. Hendrawati , W. D. Rusanti, (2020). Pengaruh penambahan gel aloe vera terhadap efektivitas antiseptik gel. Jurnal Teknologi Universitas Muhammadiyah Jakarta. 12 (1).
Talkua, Calvin., Rumengan, Yuliana. (2020). Pengaruh konsentrasi garam dan lama fermentasi terhadap kadar protein bakasang laor. Jurnal Pendidikan Biologi, 7(2),136-142.
https://doi.org/10.31849/bl.v7i2.5300
Wahyuni, R., Herliawati., Purnamasari, N. (2017). Penggunaan alkohol sebagai desinfektan pada terapi komplementer bekam. seminar dan workshop nasional keperawatan “implikasi perawatan paliatif pada bidang kesehatan”.
All articles submitted by the author and published in the Journal of Research in Pharmacy and Pharmaceutical Sciences, are fully copyrighted by the publication of JJournal of Research in Pharmacy and Pharmaceutical Sciences under the Creative Commons Attribution-NonCommercial 4.0 International License by technically filling out the copyright transfer agreement and sending it to the publisher
Note :
The author can include in separate contractual arrangements for the non-exclusive distribution of rich versions of journal publications (for example: posting them to an institutional repository or publishing them in a book), with the acknowledgment of their initial publication in this journal.
Authors are permitted and encouraged to post their work online (for example: in an institutional repository or on their website) before and during the submission process because it can lead to productive exchanges, as well as earlier and more powerful citations of published works. (See Open Access Effects).