Pengaruh Pemberian Ekstrak Buah Naga Merah (Hylocereus polyrhizus) Terhadap Gambaran Spermatogenesis Tikus Putih (Rattus norvegicus) Galur Wistar yang Diinduksi Pakan Tinggi Lemak
DOI:
https://doi.org/10.33533/jpm.v13i2.780Keywords:
Hylocereus polyrhizus, High fat diet, Spermatogenesis, Cholesterol, SimvastatinAbstract
Spermatogenesis can be affected by oxidative stress produced by the increasing of pro-inflammatory adipokines because of high fat diet.The spermatogenesis cells damaged can be prevented with natural substance such as red pitaya (Hylocereus polyrhizus). This study aims to analyze effect of Hylocereus polyrhizus extract on rat’s spermatogenesis cells number that induced by high fat diet. This study had used true-experiment design. 30 rats were divided into 5 groups. Groups 1 to 4 had done treatment for 52 days and group 5 treatment for 104 days. Group 1 Negative control (K1) administered with standard diet, group 2 Positive control (K2) had administered quail egg 10ml/KgBW, group 3 Experiment 1 (P1) had administered quail egg polyrhyzus 10ml/Kg BW and with simvastatin 0,72mg/day, group 4 Experiment 2 (P2) had administered quail egg 10ml/KgBW and wth Hylocereus polyrhizus extract 60mg/day in 52 days, and group 5 Experiment 3 (P3) had administered quail egg 10ml/KgBW and with Hylocereus polyrhizus extract 60mg/day in 104 days. Data were analyzed by One Way ANOVA and continued with Post Hoc Tukey’s HSD (P-Value=0,05) for 52 day groups. Group P1 and P2 showed repairing effect and increasing spermatocytes and spermatids (p.000<0,05) compared to group K2. Group P2 had more spermatids and spermatocytes than group P1 (p.000<0,05). Group P2 52 days’ spermatid cells equalled to group P3 104 days (p.469>0,05). Hylocereus polyrhizus extract not only had mechanism like simvastatin but also contained antioxidant, and a long-term treatment had not proven bad effect to spermatogenesis cells. Conclusion. Hylocereus polyrhizus extract 60mg/day can repair and increase spermatogenesis cells induced with high fat diet.
References
Agarwal, A, Virk, G, Ong, C, Plessis, SSD 2014, ‘Effect of Oxidative Stress on Male Reproduction’, The world journal of men’s health, 32(1), pp. 1-17, diakses tanggal 31 Juli 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026229/pdf/wjmh-32-1.pdf
Agustina 2014, Pengaruh Pemberian Kitosan terhadap Kadar Kolesterol Total Tikus (Sprague-dawley) yang diberi Pakan Tinggi Asam Lemak Trans, Skripsi, Institut Pertanian Bogor, Bogor, diakses tanggal 12 Juli 2017
http://repository.ipb.ac.id/bitstream/123456789/72032/1/I14agu.pdf
Aitken, RJ dan Roman, SD 2008, ‘Antioxidant systems and Oxidative Stress in the Testes’, pp. 154-171, diakses tanggal 31 Juli 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715191/pdf/omcl0101_0015.pdf
Anas, E dan Asterina 2011, ‘Efek Pemberian Just Tomat (Lycopersicum Pyriporme) Terhadap Spermatogenesis pada Tikus Putih (Rattus novergicus) Jantan Dewasa Hyperkholestrolemia’, Majalah Kedokteran Andalas, 35(1), hlm. 27-36, diakses tanggal 12 Juli 2017
http://jurnalmka.fk.unand.ac.id/index.php/art/article/download/88/84
Arsana, PM, Rosandi, R, Manaf, A, Budhiarta, AAG, Permana, H, Sucipta, KW, Lindarto, D, Adi, S, Pramono, B, Harbuwono, DS, Shahab, A, Sugiarto, Karimi, J, Purnomo, LB, Yuwono, A, Suhartono, T 2015, Panduan Pengelolaan Dislipidemia di Indonesia – 2015, PB. PERKENI, Indonesia, diakses tanggal 12 Juli 2017
https://puskespemda.net/download/panduan-pengelola-dislipidemia-2015-perkeni/
Ashrafi, H, Ghabili, K, Alihemmati, A, Jouyban, A, Shoja, MM, Aslanabadi, S, Adl, FH, Ghavimi, H, Hajhosseini, L 2013, ‘The Effect of Quince Leaf (Cydonia oblonga miller) Decoction on Testes in Hypercholesterolemic Rabbits : a Pilot Study’, Afr J Tradit Complement Altern Med, pp. 277-282, diakses tanggal 20 Maret 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3746575/pdf/AJT1002-0277.pdf
Aziz, FA dan Noor, MM 2010, ‘Ethanol extract of dragon fruit and its effects on sperm quality and histology of the testes in mice’, Biomedical Research, 21(2), pp. 126-130, diakses tanggal 12 Juli 2017
Bagheri, M, Nair, RR, Singh, KK, Saini, DK 2017, ‘ATM-ROS-iNOS axis regulates nitric oxide mediated cellular senescence’, Biochimica et Biophysica Acta – Molecular Cell Research, Elsevier B.V, 1864(1), pp. 1-23, diakses tanggal 31 Agustus 2017
https://www.sciencedirect.com/science/article/pii/S0167488916302944
Balitbangkes, 2013, Riset Kesehatan Dasar 2013, Kementrian Kesehatan RI, Jakarta, diakses tanggal 21 April 2017
http://www.depkes.go.id/resources/download/general/Hasil%20Riskesdas%202013.pdf
Banach, M, Rizzo, M, Toth, PP, Farnier, M, Davidson, MH, Al-Rasadi, K, Aronow, WS, Athyros, V, Djuric, DM, Ezhov, MV, Greenfield, RS, Hovingh, GK, Kostner, K, Serban, C, Lighezan, D, Fras, Z, Moriarty, PM, Muntner, P, Goudev, A, Ceska, R, Nicholls, SJ, Broncel, M, Nikolic, D, Pella, D, Puri, R, Rysz, J, Wong, ND, Bajinok, L, Jones, SR, Ray, KK, Mikhailidis, DP 2015, ‘Statin intolerance – An attempt at a unified definition. Position paper from an International Lipid Expert Panel’, Archives of Medical Science, 11(1), pp. 1-23, diakses tanggal 12 Juli 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379380/pdf/AMS-11-24816.pdf
Celik, I, Tuluce, Y, Isik, I 2007, ‘Evaluation of toxicity of abcisic acid and gibberellic acid in rats: 50 days drinking water study’, Journal of Enzyme Inhibition and Medicinal Chemistry, 22(2), pp.219-226, diakses tanggal 31 Maret 2018
https://www.tandfonline.com/doi/pdf/10.1080/14756360600988955?needAccess=true
Cheah, Y and Yang, W 2011, ‘Functions of essential nutrition for high quality spermatogenesis’, Advances in Bioscience and Biotectnology, 2(4), pp. 182-197, diakses tanggal 20 Maret 2018
https://file.scirp.org/pdf/ABB20110400019_73440528.pdf
Creswell, JW 2013, Research Design : Qualitative, Quantitative, and Mixed Methods Approaches, 4th ed, SAGE Publications, Inc. USA
Dahlan, MS 2017, Statistik untuk Kedokteran dan Kesehatan : deskriptif, bivariat, dan multivariat dilengkapi Aplikasi Mengunakan SPSS, Edisi 6, Epidemiologi Indonesia, Jakarta
Dotsch, V, Bernassola, F, Coutandin, D, Candi, E, Melino, G 2010, ‘P63 and P73, the Ancestors of P53’, Cold Harbor perspectives in biology, 2(9), pp.1-14, diakses tanggal 1 April 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926756/pdf/cshperspect-PFT-a004887.pdf
Elon, Y dan Polancos, J 2015, ‘Manfaat Jeruk Nipis (Citrus Aurantifolia) dan Olahraga untuk Menurunkan Kolesterol Total Klien Dewasa’, Jurnal Skolastik Keperawatan, 1(2), hlm. 148-155, diakses tanggal 19 September 2017
http://jurnal.unai.edu/index.php/jsk/manager/files/articles/156/public/156-541-1-PB.pdf
Erwinanto, Santoso, A, Putranto, JNE, Pradana, T, Suryawan, R, Rifqi, S, Kasiman, S 2013, Pedoman Tatalaksana Dislipidemia, Centra Communications, Indonesia, diakses tanggal 12 Juli 2017
http://www.inaheart.org/upload/file/Pedoman_tatalksana_Dislipidemia.pdf
FAO, 2010, Fats and fatty acids in human nutrition, Food and Agriculture Organization of United Nations, Rome, diakses tanggal 5 Maret 2018
http://foris.fao.org/preview/25553-0ece4cb94ac52f9a25af77ca5cfba7a8c.pdf
Guan, Y, Liang, G, Hawken, PAR, Meachem, SJ, Malecki, IA, Ham, S, Stewart, T, Guan, LL, Martin, GB 2013, ‘Nutrition affects Sertoli cell function but not Sertoli cell numbers in sexually mature male sheep’, Reproduction, Fertility, and Development, 28(8), pp. 1152-1163, diakses tanggal 20 Maret 2018
Handayani, S 2014, Kandungan Kimia beberapa tanaman dan Kulit Buah berwarna serta manfaatnya bagi Kesehatan, Universitas Negeri Yogyakarta, Yogyakarta, diakses tanggal 12 Juli 2017
http://staffnew.uny.ac.id/upload/132162017/pengabdian/ppm-kulit-buah-berwarna2014.pdf
Harika, RK, Eilander, A, Alssema, M, Osendarp, SJM, Zock, PL 2013, ‘Intake of fatty acids in general populations worldwide does not meet dietary recommendations to prevent coronary heart disease: A systematic review of data from 40 countries’, Annals of Nutrition and Metabolism, 63(3), pp. 229-238, diakses tanggal 5 Maret 2018
https://www.karger.com/Article/Pdf/355437
Hascheck, WM, Rousseaux, CG, Wallig, MA 2010 ‘Male Reproductive System’, Fundamentals of Toxicologic Phatology, pp. 553-597, diakses tanggal 29 Maret 2018
https://www.sciencedirect.com/science/article/pii/B9780123704696000180
Hor, SY, Ahmad, M, Farsi, E, Yam, MF, Hashim, MA, Lim, CP, Sadikun, A, Asmawi, MZ 2012, ‘Safety assessment of methanol extract of red dragon fruit (Hylocereus polyrhizus) : Acute and Subchronic toxicity studies’, Regulatory Toxicology and Pharmacology, Elsevier Inc., 63(1), pp. 106-114, diakses tanggal 1 April 2018
Indriasari, I 2012, Ekstrak Buah Naga Merah (Hylocereus polyrhizus) Memperbaiki Profil Lipid pada Tikus Wistar Jantan (Rattus norvegicus) Dislipidemia, Tesis, Universitas Udayana, Denpasar, diakses tanggal 12 Juli 2017
https://anzdoc.com/ekstrak-ethanol-buah-naga-merah-hylocereus-polyrhizus-memper.html
Istianah, ET 2016, Perbedaan Kadar Asam Urat pada Pasien Tidak Puasa dengan Pasien Puasa 8, 10, dan 12 Jam, Skripsi, Universitas Muhammadiyah, Semarang, diakses tanggal 19 Maret 2018
http://repository.unimus.ac.id/122/1/SKRIPSI.pdf
Jan, SZ, Jongejan, A, Korver, CM, Daalen, SKMV, Pelt, AMMV, Repping, S, Hamer, G 2018, ‘Distinct prophase arrest mechanisms in human male meiosis’, The Company of Biologists Ltd, diakses tanggal 29 Maret 2018
http://dev.biologists.org/content/early/2018/03/13/dev.160614.full.pdf
Kim, HY 2015, ‘Statistical notes for clinical researchers : post-hoc multiple comparisons’, Restorative Dentistry & Endodontics, 40(2), pp. 172-176, diakses tanggal 4 April 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432262/pdf/rde-40-172.pdf
Kristanto, D 2014, Berkebun Buah Naga, Penebar Swadaya, Jakarta
Kusuma, AM, Asarina, Y, Rahmawati, YI, Susanti 2016, ‘Efek Ekstrak Bawang Dayak (Eleutherine palmifolia (L.) Merr) dan Ubi Ungu (Ipomoea batatas L) terhadap Penurunan Kadar Kolesterol dan Trigliserida Darah pada Tikus Jantan’, Jurna Kefarmasian Indonesia, 6(2), hlm. 108-116, diakses tanggal 12 Juli 2017
https://media.neliti.com/media/publications/105793-ID-efek-ekstrak-bawang-dayak-eleutherine-pa.pdf
Mahattanatawee, K, Manthey, JA, Luzio, G, Talcott, ST, Goodner, K, Baldwin, EA 2006, ‘Total antioxidant activity and fiber content of select Florida-Grown Tropical Fruits’, Journal of Agricultural and Food Chemistry, 54(19), pp. 7355-7363, diakses tanggal 12 Juli 2017
https://pdfs.semanticscholar.org/1e55/e97b963314845fb5e4583dd0725f1e07eb17.pdf
Murray, RK, Bender, DA, Botham, KM, Kennelly, PJ, Rodwell, VW, Weil, PA 2009, Haper’s Illustrated Biochemistry, 28th ed, The McGraw-Hill Companies, Inc. USA
Musfiroh, M dan Gustari, S 2015, ‘Pengaruh pemberian minyak jintan hitam (Nigella sativa L.) terhadap peningkatan spermatogenesis tikus wistar yang terpapar asap rokok’, Jurnal Kedokteran Hewan, 9(2), hlm. 114-116, diakses tanggal 29 September 2017
Opie, LH 2014, ‘Lifestyle and diet : review article’, Cardiovascular Journal of Africa, 25(6), pp. 298-301, diakses tanggal 5 Maret 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336914/pdf/cvja-25-298.pdf
Ouchi, N, Parker, JL, Lugus, JJ, Walsh, K 2012, ‘Adipokines in inflammation and metabolic disease’, NIH Public Access. 11 (2), diakses tanggal 2 Agustus 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518031/pdf/nihms420556.pdf
Parasuraman, S 2011, ‘Toxicological screening’, Journal of Pharmacology and Pharmacotherapeutics, 2(2), pp. 74-79, diakses tanggal 31 Maret 2018
http://www.jpharmacol.com/temp/JPharmacolPharmacother2274-3904373_105043.pdf
Peltzer, K 2018, ‘Prevalence, risk awareness and health beliefs of behavioural risk factors for cardiovascular disease among university students in nine ASEAN countries’, BMC Public Health, pp.1-9, diakses tanggal 5 Maret 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810026/pdf/12889_2018_Article_5142.pdf
Prakoso, LO 2017, Perbedaan Efek Ekstrak Buah Naga Merah (Hylocereus polyrhizus) dan Ekstrak Buah Naga Putih (Hylocereus undatus) Terhadap Penurunan Kadar Kolesterol Total pada Tikus Putih (Rattus novergicus), Skripsi, UPN Veteran Jakarta, Jakarta
Pushpendra, A dan Jain, GC 2015, ‘Hyper-Lipidemia and Male Fertility: A Critical Review of Literature’, Andrology-Open Access, 4(2), diakses tanggal 22 April 2017
Reece, JB, Urry, LA, Cain, ML, Wasserman, SA, Minorsky, PV, Jackson, RB 2011, Campbell Biology, 9th ed, Pearson Education, Inc, USA
Sastroasmoro, S dan Ismael, S 2011, Dasar-dasar Metodologi Penelitian Klinis, Edisi 4, Sagung Seto, Jakarta
Sharan 2017, ‘Ekstrak etanol buah naga merah (Hylocereus polyrhizus) memperbaiki profil lipid tikus (Rattus novergicus) wistar jantan dislipidemia sama efektif dengan statin’, Intisari Sains Medis, 8(2), hlm. 102-109, diakses tanggal 31 Agustus 2017
https://isainsmedis.id/index.php/ism/article/download/118/137
Shi, Y, Guo, R, Wang, X Yuan, D, Zhang, S, Wang, J, Yang, X, Wang, C 2014, ‘The regulation of alfalfa saponin extract on key genes involved in hepatic cholesterol metabolism in hypelipidemic rats’, PLOS ONE, 9(2), diakses tanggal 19 September 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914959/pdf/pone.0088282.pdf
Singh, NU, Roy, A, Tripathi, AK 2013, ‘Non Parametric Tests : Hands on SPSS’, ICAR Reseearch Complex fo NEH Region, Meghalaya, diakses tanggal 8 September 2017
http://www.kiran.nic.in/pdf/Social_Science/e-learning/Non_Parametrict_Test.pdf
Sutanto, PH 2006, Analisis Data, Universitas Indonesia, Depok
Widianingsih, M 2016, ‘Aktivitas antioksidan ekstrak metanol buah naga merah (Hylocereus polyrhizus (F. A. C. Weber) Britton & Rose) Hasil Maserasi dan dipekatkan dengan kering angin’, Jurnal Wiyata, 3(2), hlm. 146-150, diakses tanggal 31 Agustus 2017
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