Keywords
Staphylococcus aureus
Propionibacterium acnes
antimicrobial
How to Cite
Abstract
Acne vulgaris is an inflammatory disorder of pilosebaceous glands caused by an increase in sebum production, abnormal follicle keratinization, and colonization of positive gram P. acnes and S. epidermidis bacteria. Clindamycin antibiotic resistance has been reported because it is used to bacteria that cause acne vulgaris. Garlic extract has antimicrobial properties called allicin. This study aimed to determine the in vitro antimicrobial effectiveness of garlic extract in the growth of P. acnes and S. epidermidis. The samples of this study are garlic extract. This study was a true experimental study conducted using disc diffusion method with Mueller Hinton Agar. The zone of inhibition was observed on all groups of P. acnes and S. epidermidis in all tested concentrations with allicin concentration of 256µg/ml showing the widest zone of inhibition. Statistical analysis using the One- Way ANOVA resulted in (p)<0.005. Conclusively, garlic extract was effective in inhibiting the growth of P. acnes and S. epidermidis.
References
Kirby-Bauer Disk Diffussion Susceptibility Test Protocol, diakses 09 Juni2018,https://www.asm.org/getattachment/2594ce26-bd44-47f6-82870657aa9185ad/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test- Protocol-pdf.pdf
Astal, ZE 2004, ‘The Inhibitory Action of Aqueous Garlic Extract on the Growth of Certain Pathogenic Bacteria’, European Food Research and Technology, vol.218, no.5, hlm 460-464, diakses 22 Desember 2018 https://link.springer.com/article/10. 1007/s00217-003-0864-3
Borlinghaus, J, Albrecht, F, Gruhlke, MCH, Nwachukwu, ID, Slusarenko, AJ 2014, ’Allicin: Chemistry and Biological Properties’, Molecules, vol.19, no.8, hlm 12591-12618, diakses 22 Mei 2019 https://www.ncbi.nlm.nih.gov/pubmed/25153873
Chandani, KU, Raval, RC, Rana, DA, Malhotra, SD 2018, ‘Study of Drug Use Pattern & Analysis of Quality of Life In Patients of Acne Attending The Dermatology OPD In A Tertiary Care Hospital’, NHL Medical Municipal College, vol.9, no.1, hlm 108-116, diakses 22 Mei 2019 http://nicpd.ac.in/ojs/index.php/njirm/article/download/1 864/1686/
Charles, F, Sanchez, J, Gontard, N 2006, ‘Absorption Kinetics of Oxygen and Carbon Dioxide Scavengers as Part of Active Modified Atmosphere Packaging’, Journal of Food Engineering, vol.72, no.1, hlm 1-7, diakses 19 April 2019 https://www.sciencedirect.com/science/article/abs/pii/S026087740400585 0?via%3Dihub
Chong, K, Zamora, MP, Tilakawardane, DA, Buckley, NE, Rego, JA, Liu, Y 2014, ‘Investigation of Allicin Stability in Aqueous Garlic Extract by High Performance Liquid Chromatography Method’, Journal of Scientific Research & Reports, vol.4, no.7, hlm 590-598, diakses 19 April 2019 https://www.researchgate.net/publication/282624373_Investigation_of_Allicin_Stability_in_Aqueous_GarlicExtract_by_High_Performance_Liqu id_Chromatography_Method
Damayanti, M 2014, ‘Uji Efektivitas Larutan Bawang Putih (Allium sativum) terhadap Pertumbuhan Bakteri Propionibacterium acnes secara in vitro’, Skripsi Program Studi Pendidikan Dokter, UIN Syarif Hidayatullah Jakarta, diakses 22 Mei 2019http://repository.uinjkt.ac.id/dspace/bitstream/123456789/27214/1/MAY A%20DAMAYANTI-FKIK.pdf
Davis, WW, Stout, TR 1971, ‘Disc Plate Method of Microbiological Antibiotic Assay’, American Society for Microbiology, vol.22, no.4, hlm 659-665, diakses 12 Januari 2019 https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC376382/
Dreno, B, Martin, R, Moyal, D, Henley, JB, Khammari, A, Seite, S 2016, ‘Skin Microbiome and Acne Vulgaris: Staphylococcus, a New Actor in Acne’, Wiley Experimental Dermatology, vol.26, no.9, hlm 798- 803, diakses 12 Januari 2019 https://www.ncbi.nlm.nih.gov/pubm ed/28094874
Marchese, A, Barbieri, R, Silva, AS, Daglia, M, Nabavi, SF, Jafari, NJ, Izadi, M, Ajami, M, Nabavi, SM 2016, ‘Antifungal and Antibacterial Activities of Allicin: a Review’, Trends in Food Science & Technology, vol.52, no.16, hlm 49- 56, diakses 5 Juni 2019 https://www.sciencedirect.com/scie nce/article/abs/pii/S0924224416300 073
McDade, JJ, Sabel, FL, Akers, RL, Walker, RJ 1968, ‘Microbiological Studies on the Performance of a Laminar Airflow Biological Cabinet’, American Society for Microbiology, vol.16, no.7, hlm 1086-1092, diakses
April 2019
https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC547593/
Morris-Jones, R 2014, ABC of Dermatology, Edisi 6, Blackwell, United Kingdom
Murray, RP, Rosenthal, KS, Pfaller, MA 2016, Medical Microbiology, Edisi 8, Elsevier, Philadelphia
Nabatanzi, A 2018, ‘In-vitro Antibacterial Activity of Allium sativum L. Clove Extract Against Agrobacterium tumefaciens’, Science Domain International, vol.16, no.6, hlm 1-7, diakses 7 Juni 2019
https://www.researchgate.net/public ation/328516055_In- vitro_Antibacterial_Activity_of_All ium_sativum_L_Clove_Extract_Ag ainst_Agrobacterium_tumefaciens
Rahman, MM, Fazlic, V, Saad, NW 2012, ‘Antioxidant Properties of Raw Garlic (Allium sativum) Extract’, International Food Research Journal, vol.19, no.2, hlm 589-591, diakses 30 April 2019
http://www.ifrj.upm.edu.my/19%20 (02)%202012/(32)IFRJ-
%20Rahman.pdf
Santer, M, Francis, NA, Platt, D, Eady, EA, Layton, AM 2018, ‘Stemming the Tide of Antimicrobial Resistance: Implications for Management of Acne Vulgaris’, British Journal of General Practice, vol.68, no.667, hlm 64-65, diakses 10 Mei 2019 https://www.ncbi.nlm.nih.gov/pubm ed/29371295
Saxena, G, Sadawarte, K, Kaore, NM 2018, ‘Antibacterial Activity of Aqueous Extract of Garlic (Allium sativum) on Standard Strains’, Journal of Evolution of Medical and Dental Sciences, vol.7, no.19, hlm 2320- 2322, diakses 22 Mei 2019 https://www.researchgate.net/publication/327950658_ANTIBACTERIAL_ACTIVITY_OF_AQUEOUS_EXTRACT_OF_GARLIC_ALLIUM_SATIVUM_ON_STANDARD_STRAI NS
Sinaga, E 2004, Infeksi Nosokomial dan Staphylococcus epidermidis, EGC, Jakarta
Tan, JKL, Bhate, K 2015, ‘A Global Perspective on the Epidemiology of Acne’, British Journal of Dermatology, vol. 172, no.51, hlm 3–12, diakses 22 Mei 2019 https://onlinelibrary.wiley.com/doi/ pdf/10.1111/bjd.13462
Lawal, B, Shittu, OK, Oibiokpa, FI, Mohammed, H, Umar, SI, Haruna, GM 2016, ‘Antimicrobial Evaluation, Acute and Sub-acute Toxicity Studies of Allium sativum’, Journal of Acute Disease, vol.5, no.4, hlm 296-301, diakses 19 April 2019https://www.sciencedirect.com/science/article/pii/S2221618916300592
The Monthly Index of Medical Specialities 2018, Clindamycin + Benzoyl peroxide, diakses pada 18 Februari 2019,
Tjekyan, RMS 2008, ‘Kejadian dan Faktor Resiko Akne Vulgaris’, Jurnal Kedokteran Media Medika Indonesia, vol.43, no.1, hlm 37-43, diakses 5 Juni 2019https://ejournal.undip.ac.id/index.ph p/mmi/article/view/3810
Williams, HC, Dellavalle, RP, Garner, S 2012, ‘Acne Vulgaris’, diakses Juli 2018.https://www.thelancet.com/journals/l ancet/article/PIIS0140- 6736(11)60321-8/fulltext
World Health Organization 2017, ‘Acne vulgaris’, diakses pada 18 Februari 2019,http://apps.who.int/medicinedocs/en/ d/Jh2918e/20.html
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