Keywords
Dilution
Disk method
Pseudomonas aeruginosa
Staphylococcus aureus
How to Cite
Abstract
The susceptibility test is a test used to measure bacteria's sensitivity and vulnerability towards antibiotics. This study was to determine sensitivity of Pseudomonas aeruginosa and Staphylococcus aureus towards amoxicillin, neomycin, and sulfanilamide. In this study, the methods used in susceptibility tests are disk diffusion method and serial dilution. The disk diffusion method is a method with paper disks that already saturated the antibiotics, with one paper disk for each antibiotic, put the disk on the agar media that had been inoculated by the bacteria, then incubated and measured the inhibition zone. The dilution method was done by making a series of antibiotic dilutions in liquid media which added microbes inside. This test is to estimate the smallest concentration or Minimum Inhibitory Concentration (MIC) in the dilution series with no bacterial growth. The diffusion test shows no clear visible zone at all, so Pseudomonas aeruginosa and Staphylococcus aureus are resistant to amoxicillin, neomycin and sulfanilamide. The dilution test shows the clearest sample was on 0,25% concentrate, so amoxicillin's Minimum Inhibitory Concentration towards Staphylococcus aureus is 0,25%.
References
Astutiningsih, C., Setyani, W., & Hindratna, H. (2014). Uji daya antibakteri dan identifikasi isolat senyawa katekin dari daun teh (Camellia Sinensisl. Var Assamica). Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 11(2).
Aviany, H. B., & Pujiyanto, S. (2020). Analisis Efektivitas Probiotik di Dalam Produk Kecantikan sebagai Antibakteri terhadap Bakteri Staphylococcus epidermidis. Berkala Bioteknologi, 3(2).
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis, 6(2), 71-79.
Bernatová, S., Samek, O., Pilát, Z., Šerý, M., Ježek, J., Jákl, P., ... & Růžička, F. (2013). Following the mechanisms of bacteriostatic versus bactericidal action using Raman spectroscopy. Molecules, 18(11), 13188-13199.
Fitriana, Y. A. N., Fatimah, V. A. N., & Fitri, A. S. (2020). Aktivitas anti bakteri daun sirih: uji ekstrak KHM (Kadar Hambat Minimum) dan KBM (Kadar Bakterisidal Minimum). Sainteks, 16(2).
Intan, K., Diani, A., & Nurul, A. S. R. (2021). Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kesehatan Perintis, 8(2), 121-127.
Krause, K. M., Serio, A. W., Kane, T. R., & Connolly, L. E. (2016). Aminoglycosides: an overview. Cold Spring Harbor perspectives in medicine, 6(6).
Khusuma, A., Safitri, Y., Yuniarni, A., & Rizki, K. (2019). Uji Teknik Difusi Menggunakan Kertas Saring Media Tampung Antibiotik dengan Escherichia Coli Sebagai Bakteri Uji. Jurnal Kesehatan Prima, 13(2), 151-155.
Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan pengujian aktivitas antibakteri starter yogurt dengan metode difusi sumuran dan metode difusi cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41-46.
Murtafi’ah, dkk. (2023). Bakteriologi 1. Padang: Get Press Indonesia.
Murwani, S. (2015). Dasar-dasar Mikrobiologi veteriner. Universitas Brawijaya Press.
National Center for Biotechnology Information. 2023. PubChem Compound Summary for CID 33613, Amoxicillin. Retrieved November 20, 2023 from Amoxicillin | C16H19N3O5S | CID 33613 - PubChem.
Pelczar, M. J. (2019). Dasar-dasar mikrobiologi.
Prayoga, E. (2013). Perbandingan Efek Ekstrak Daun sirih hijau (Piper betle L.) dengan metode difusi disk dan sumuran terhadap pertumbuhan bakteri Staphylococcus aureus.
Rahman, I. W., & Prihartini, A. (2021). Uji Sensitivitas Antibiotik terhadap Pertumbuhan Klebsiella pneumonia dari Sputum Penderita Infeksi Saluran Pernapasan Bawah. Journal of Health Education Economics Science and Technology (J-HEST), 3(2), 81-87.
Soleha, T. U. (2015). Uji kepekaan terhadap antibiotik. Juke Unila, 5(9), 119-123.
Tjay, T. H., & Rahardja, K. (2007). Obat-obat penting: khasiat, penggunaan dan efek-efek sampingnya. Elex Media Komputindo.
Zessel, K., Mohring, S., Hamscher, G., Kietzmann, M., & Stahl, J. (2014). Biocompatibility and antibacterial activity of photolytic products of sulfonamides. Chemosphere, 100, 167-174.
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Copyright (c) 2023 Raffi Joe Bhagaskara, Alyaa Shofura Ahmad, Syifa Regina Prasetyawan, Dewi Amelia, Deri Putra Pratama, Innayah Marda Hidayyah Tulloh, Naadhira Alya Putri, Indira Damayanti Putri, Alya Layyina Rabbani, Fathan Mubiin Ibnu Ismail, Jihan Tria Astuti, Naufal Brian Atmaja