Differences in Corpse Stiffness (Rigor Mortis) of Organophosphate-Induced and Ordinary (Decerebrated) Dead Wistar Rats : A Randomized Experimental Study

Authors

  • Novianto Adi Nugroho Fakultas Kedokteran, Univesitas Sebelas Maret, Surakarta, Indonesia https://orcid.org/0009-0001-6122-4337
  • Arzaq Fikrian Zahrawan Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, Indonesia
  • Hari Wujoso Fakultas Kedokteran, Univesitas Sebelas Maret, Surakarta, Indonesia https://orcid.org/0000-0003-0360-5876
  • Wahyu Dwi Atmoko RSUD Dr.Moewardi, Surakarta, Indonesia

DOI:

https://doi.org/10.33533/jpm.v18i2.9215

Keywords:

Organophosphates, Poisoning, Corpse Stiffness, Rigor Mortis

Abstract

Corpse Stiffness (rigor mortis) is a secondary sign of death that can be used to estimate the time and cause of death. Organophosphates are the most toxic pesticides and often cause poisoning until death in humans. This study aims to determine the differences in corpse stiffness (rigor mortis) due to organophosphate poisoning and ordinary death (decerebration) using Wistar rats. The parameters of corpse stiffness (rigor mortis) used include 4 aspects: Corpse Stiffness Appearance, Perfect Formation of Corpse Stiffness, Persistent Corpse Stiffness, and Relaxation of Corpse Stiffness. Data were processed with univariate analysis and then tested with the Independent Sample T-test. There was a significant difference (p<0.05) in the duration of the appearance, perfect formation, persistent and relaxation of corpse stiffness between the control group and the treatment group. Meanwhile, there was no significant difference (p>0.05) in persistent corpse stiffness duration between the control and treatment groups, although there was a slight difference in the mean. Between ordinary and died rats due to organophosphate induction, there was a significant difference in the duration of appearance, perfect formation, and relaxation of corpse stiffness. However, there was no significant difference in the duration of persistent stiffness.

Keywords: Organophosphates; Poisoning; Corpse Stiffness; Rigor Mortis

Author Biographies

Novianto Adi Nugroho, Fakultas Kedokteran, Univesitas Sebelas Maret, Surakarta, Indonesia

Departemen Forensik dan Medikolegal

Hari Wujoso, Fakultas Kedokteran, Univesitas Sebelas Maret, Surakarta, Indonesia

Departemen Forensik dan Medikolegal

Wahyu Dwi Atmoko, RSUD Dr.Moewardi, Surakarta, Indonesia

Instalasi Kedokteran Forensik dan Medikolegal

References

Boedeker W, Watts M, Clausing P, Marquez E. The global distribution of acute unintentional pesticide poisoning: Estimations based on a systematic review. BMC Public Health. 2020;20(1). DOI: https://doi.org/10.1186/s12889-020-09939-0

Badan Pusat Statistik. Keadaan Angkatan Kerja di Indonesia Februari 2023. Jakarta, Indonesia: Badan Pusat Statistik; 2023.

Wu X, Li J, Zhou Z, Lin Z, Pang S, Bhatt P, et al. Environmental occurrence, toxicity concerns, and degradation of Diazinon using a microbial system. Frontiers in Microbiology. 2021;12. DOI: https://doi.org/doi:10.3389/fmicb.2021.717286

Yadav, Ishwar & Devi, Ningombam. Pesticides classification and its impact on human and environment. Environmental science and engineering. LCC, USA: Studium Press ; 2017. (7; vol. 6).

Amir A, Raza A, Qureshi T, Mahesar GB, Jafferi S, Haleem F, et al. Organophosphate poisoning: Demographics, severity scores and outcomes from National Poisoning Control Centre, Karachi. Cureus. 2020; DOI: https://doi.org/doi:10.7759/cureus.8371

Tyler J, Kris B, Roshana S, Anmol S, Shaza A. Organophosphate poisoning and suicide in Nepal: A reflection on the limitations of Behavioral Health Resources. International Journal of Critical Care and Emergency Medicine. 2020;6(1). DOI: https://doi.org/doi:10.23937/2474-3674/1510097

van Zyl-Smit RN, Richards G. Organophosphate poisoning – shadow-boxing in the intensive care unit. Afr J Thorac Crit Care Med. 2019 Sept 17;25. DOI: https://doi.org/10.0.28.28/AJTC-CM.2019.v25i3.025.

Hulse EJ, Haslam JD, Emmett SR, Woolley T. Organophosphorus nerve agent poisoning: Managing the Poisoned patient. British Journal of Anaesthesia. 2019;123(4):457–63. DOI: https://doi.org/10.1016/j.bja.2019.04.061

van Melis LVJ, Heusinkveld HJ, Langendoen C, Peters A, Westerink RHS. Organophosphate insecticides disturb neuronal network development and function via non-ache mediated mechanisms. NeuroToxicology. 2023;94:35–45. DOI: https://doi.org/10.1016/j.neuro.2022.11.002

Shrestha R, Kanchan T, Krishan K. Methods of estimation of time since death [Internet]. U.S. National Library of Medicine; 2023 [cited 2023 Sept 24]. Available from: https://pubmed.ncbi.nlm.nih.gov/31751057/

Shedge R, Krishan K, Warrier V, Kanchan T. Postmortem changes - statpearls - NCBI bookshelf [Internet]. StatPearls Publishing; 2023 [cited 2023 Sept 24]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK539741/

Parinduri AG. Buku Ajar Kedokteran Forensik dan Medikolegal. Medan, Indonesia: UMSU Press; 2020. ISBN/ISSN. 978-602-6997-99-9

Almulhim AM, Menezes RG. Evaluation of postmortem changes [Internet]. U.S. National Library of Medicine; 2023 [cited 2023 Sept 24]. Available from: https://pubmed.ncbi.nlm.nih.gov/32119351/

Fitria L. Profil hematologi Tikus (rattus Norvegicus Berkenhout, 1769) Galur Wistar jantan Dan Betina umur 4, 6, Dan 8 Minggu. Biogenesis: Jurnal Ilmiah Biologi. 2014;2(2):94–100. DOI: https://doi.org/10.24252/bio.v2i2.473

Bhadaniya AR, Kalariya VA, Joshi DV, Patel BJ, Chaudhary S, Patel HB, et al. Toxicopathological evaluation in wistar rats (rattus norvegicus) following repeated oral exposure to acephate. Toxicol Ind Health. 2015;31(1):9–17. DOI: https://doi.org/10.1177/0748233712468017

Kupferschmid BJ, Therrien B, Rowsey PJ. Different sickness responses in adult and aged rats following lipopolysaccharide administration. Biol Res Nurs. 2018;20(3):335–342. DOI: https://doi.org/10.1177/1099800418759599

Nair A, Jacob S. A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy. 2016;7(2):27. DOI: https://doi.org/10.4103/0976-0105.177703

Kim HY. Statistical notes for clinical researchers: The independent samples t-test. Restor Dent Endod. 2019;44(3):e26. DOI: https://doi.org/10.5395/rde.2019.44.e26

Green, D. R., & Llambi, F. Cell Death Signaling. Cold Spring Harbor perspectives in biology, 2015;7(12). DOI: https://doi.org/10.1101/cshperspect.a006080

Ung, Sosthène P.-M.; Li, Chao-Jun. From rocks to bioactive compounds: a journey through the global P( v ) organophosphorus industry and its sustainability. RSC Sustainability. 2023; 1 (1): 11–37. DOI: https://doi.org/10.1039/D2SU00015F

Bhatt, P., Zhou, X., Huang, Y., Zhang, W., & Chen, S. Characterization of the role of esterases in the biodegradation of organophosphate, carbamate, and pyrethroid pesticides. Journal of hazardous materials. 2021; 411, 125026. DOI: https://doi.org/10.1016/j.jhazmat.2020.125026

Naughton, S. X., & Terry, A. V., Jr. Neurotoxicity in acute and repeated organophosphate exposure. Toxicology. 2018;408, 101–112. DOI: https://doi.org/10.1016/j.tox.2018.08.011

Yan, Z., Jin, X., Liu, D., Hong, Y., Liao, W., Feng, C., & Bai, Y. The potential connections of adverse outcome pathways with the hazard identifications of typical organophosphate esters based on toxicity mechanisms. Chemosphere. 2021;266, 128989. https://doi.org/10.1016/j.chemosphere.2020.128989

Aman S, Paul S, Chowdhury FR. Management of organophosphorus poisoning. Critical Care Clinics. 2021;37(3):673–86. DOI: https://doi.org/10.1016/j.ccc.2021.03.011

Tsokos M, Byard RW. Post mortem changes - overview. In: Encyclopedia of Forensic and Legal Medicine. Academic Press. 2016 Jan;4:10–31. DOI: https://doi.org/10.1016/B0-12-369399-3/00091-4

Shivpoojan K. Time since Death from Rigor Mortis: Forensic Prospective. J Forensic Sci & Criminal Inves 2018; 9(5): 555771. DOI: https://doi.org/10.19080/JFSCI.2018.09.555771

Thanos, C. A. A., Tomuka, D., & Mallo, N. T. S. Livor mortis pada keracunan insektisida golongan organofosfat di kelinci. E-CliniC, 2016;4(1). DOI : https://doi.org/10.35790/ecl.4.1.2016.10827

Jales, J. T., Barbosa, T. M., Soares, V. P., & Gama, R. A. Effect of Terbufos (Organophosphate) on the Cadaveric Colonization Process: Implications for Postmortem Interval Calculation. Journal of medical entomology. 2021;58(3), 1056–1063. DOI: https://doi.org/10.1093/jme/tjaa284

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Published

2024-12-18

How to Cite

Nugroho, N. A., Zahrawan, A. F., Wujoso, H., & Atmoko, W. D. (2024). Differences in Corpse Stiffness (Rigor Mortis) of Organophosphate-Induced and Ordinary (Decerebrated) Dead Wistar Rats : A Randomized Experimental Study. Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 18(2), 170–179. https://doi.org/10.33533/jpm.v18i2.9215

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Vol. 18 No. 2 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan (In Press)

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