Potential of Rambutan-Honey Antioxidants in Reducing Malondialdehyde Levels and Regenerating Hepatocyte Cells in Isoniazid-Rats Induced
DOI:
https://doi.org/10.33533/jpm.v18i1.7713Keywords:
Hepatocyte cell, honey, malondialdehydeAbstract
Hepatotoxicity induced by prolonged administration of antituberculosis drugs such as isoniazid (INH) is associated with the production of free radicals. Rambutan-honey, rich in catalase enzyme, flavonoids, and vitamin C, exhibits antioxidant and immunomodulatory properties. This study purpose to assess the effect of rambutan-honey on malondialdehyde (MDA) levels and hepatocyte cell numbers in INH-induced rats. The research method using in vivo experimental study with a randomized posttest-only control group design. Thirty Wistar rats were divided into five groups: negative control(G1), positive control(G2), and three groups treated with rambutan-honey at doses of 250 mg/kgBW(G3), 500 mg/kgBW(G4), and 1000 mg/kgBW(G5). MDA levels were measured using the TBARS method on days 15 and 23, while hepatocyte cell numbers were assessed through histopathological examination using Hematoxylin-Eosin. Data were analyzed using One-Way ANOVA followed by Post-Hoc test. This study revealed that rambutan-honey at doses of 250, 500, and 1000 mg/kgBW significantly reduced MDA levels compared to controls, with a simultaneous increase in hepatocyte cell numbers (p=0.001). The conclusion of this research is the potential of rambutan-honey as an antioxidant and hepatoprotective agent, possibly mediated by its flavonoid content, which may stimulate superoxide dismutase activity akin to catalase enzyme and vitamin C.
References
Centers for disease control. Transmission and pathogenesis of tuberculosis.2016;7. Available from: https://www.cdc.gov/tb/education/corecurr/pdf/chapter2.pdf. 2016. "Accessed on [date]" 3 March 2024.
Schaberg T, Rebhan K, Lode H. Risk factors for side-effects of isoniazid, rifampin and pyrazinamide in patients hospitalized for pulmonary tuberculosis.
Eur Respir J. 1996; Risk factors for side-effects of isoniazid, rifampin and pyrazinamide in patients hospitalized for pulmonary tuberculosis 9(10):2026-30.
DOI: https://doi.org/10.1183/09031936.96.09102026
Zaw et. al. KatG 315 Mutation as a molecular determinant for isoniazid resistance in mycobacterium tuberculosis. Borneo Journal of Medical Sciences (BJMS). 2017; 11(1):1-12. DOI:
https://doi.org/10.51200/bjms.v11i2.602
Yuslianti. ER. Pengantar radikal bebas dan antioksidan. DeePublish. 2017; 109. ISBN 978-602-453-456-1
Gaglani et.al. A pro-oxidant property of vitamin C to overcome the burden of latent Mycobacterium tuberculosis infection: A cross-talk review with Fenton reaction. Front. Cell. Infect. Microbiol. 2023; Vol 13. DOI:
https://doi.org/10.3389/fcimb.2023.1152269
Yuslianti ER., Bachtiar BM., Sujiatmo AB., Suniarti DF., Sutjiatmo AB. Effect topical rambutan honey pharmaceutical grade on oral mucosa wound healing based on tissue wound closure and fibroblasts proliferation in vivo. International Journal of Pharmacology. 2015; 11 (7): 864-869. DOI: https://doi.org/10.3923/ijp.2015.864.869
Yuslianti ER, Bachtiar BM, Suniarti DF, Sutjiatmo AB. Effect of rambutan honey (Nephelium lappaceum) acute administration on mortality, body weight, toxicity symptoms and relative organ weight of Swiss webster mice. Research Journal of Medicinal Plants. 2016; 8 (1): 1-7. DOI: https://doi.org/10.3923/rjt.2016.1.7
Samarghandian S, Farkhondeh T, Samini F. Honey and health: A review of recent clinical research. Pharmacognosy Res. 2017; 9(2):121-127. DOI: https://doi.org/10.4103/0974-8490.204647.
Chaudhary P et.al. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front. Chem. Sec. Medicinal and Pharmaceutical Chemistr. 2023; Volume 11:1-24. DOI: https://doi.org/10.3389/fchem.2023.1158198
Ruan L., Fan J. T., Hong W, Zhao H, Li MH, Jiang L, Xing Wang JS. Isoniazid-induced hepatotoxicity and neurotoxicity in rats investigated by HNMR based metabolomics approach 2018. "Accessed on [date]" 15 March 2024. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29936297.
Rachmat I. I, Yuslianti E, Permatasari F, Koswara T. Antihyperglycemic effect of rambutan honey in alloxan-induced diabetic wistar rats. Research Journal of Toxicology. 2017; 12(1): 42-49. DOI: https://doi.org/10.3923/jpt.2017.42.49
Luan F, Peng L, Lei Z, Jia X, Zou J, Yang Y, He X, Zeng N. Traditional uses, phytochemical constituents and pharmacological properties of Averrhoa carambola L. A Review. Front Pharmacol. 2021 Aug 12;12:699899. DOI: https://doi.org/10.3389/fphar.2021.699899
Dewi, N.K.S.M.; Ramona, Y.; Saraswati, M.R.; Wihandani, D.M.; Wirasuta, I.M.A.G. The Potential of the Flavonoid Content of Ipomoea batatas L. as an Alternative Analog GLP-1 for Diabetes Type 2 Treatment-Systematic Review. Metabolites 2024, 14, 29. DOI: https://doi.org/10.3390/metabo14010029
Hassan HM, Yousef BA, Guo H, Xiaoxin L, Zhang L, Jiang Z. Investigating the CYP2E1 potential role in the mechanism behind INH/LPS-induced hepatotoxicity. 2018; 9: 198.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5850051/. "Accessed on [date]" 15 March 2024. DOI: https://doi.org/10.3389/fphar.2018.00198
Cordiano, R., Di Gioacchino, M., Mangifesta, R. Panzera, C. Gangemi, S., Minciullo, P.L. Malondialdehyde as a potential oxidative stress marker for allergy-oriented diseases.: An update. Molecules. 20238:, 5979. DOI: https://doi.org/10.3390/molecules28165979
Vona, R; Pallotta, L; Cappelletti, M; Severi, C; Matarrese, P. The impact of oxidative stress in human pathology: focus on gastrointestinal disorders. AAntioxidants. 2021;, 10, 201. DOI: https://doi.org/10.3390/antiox10020201
Ilesanmi OB., Adeogun EF., Odewale TT., Chikere B. Lead exposure-induced changes in hematology and biomarkers of hepatic injury: protective role of TrévoTM supplement. Environ Anal Health Toxicol. 2022 Jun;37(2):e2022007-0. doi: DOI: https://doi.org/10.5620/eaht.2022007
Ullah A., Munir S., Badshah SL., Khan N., Ghani L., Poulson BG., Emwas AH., Jaremko M. Important flavonoids and their role as a therapeutic agent. Molecules. 2020 Nov 11; 25(22):5243. DOI: https://doi.org/10.3390/molecules25225243
Peng et,al. Protective effects and mechanisms of flavonoids in renal ischemia-reperfusion injury. Pharmacology. 2023;. 1087(1): 27-36. DOI: https://doi.org/10.1159/000527262
Dias MC., Pinto DCGA., Silva AMS. Plant flavonoids: Chemical characteristics and biological activity. Molecules. 2021 Sep 4; 26(17):5377. DOI: https://doi.org/10.3390/molecules26175377
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