Testing The Antibacterial Effectiveness of Black Cumin Extract on The Growth of Bacteria Bacillus Cereus Disk Diffusion Method
DOI:
https://doi.org/10.33533/jpm.v18i1.7682Keywords:
BLACK CUMIN EXTRACT, Disk Diffusion Method, bacteriaAbstract
The main components of volatile oils are thymoquinone, thymohydroquinone, thymoquinone, thymol, and tannin which have been proven to be able to inhibit bacterial growth. This research was conducted to determine the antibacterial effectiveness of black cumin against the growth of Bacillus cereus bacteria with various concentrations of 10%, 25%, 50%, 75%, and 100% using the diffusion method. The type of research used by researchers is experimental. In this study, the disk disc method was used with concentrations of 10% 25%, 50%, 75%, and 100% using Amoxicillin 0.3% as a positive control for Bacillus cereus bacteria with repetition three times, by soaking the disc for 15 minutes in each concentration and attach the disc to media that has been inoculated with Bacillus cereus bacteria. Based on research results from the results of measurements of the inhibitory zone of the Bacillus cereus bacterium, it is included in the weak category with all concentrations showing an inhibitory zone with an average diameter of 8.67 mm, 10.67 mm, 12 mm, 16.33 mm, and 17.67 mmm. The conclusion from research on the antibacterial effectiveness of black cumin extract can inhibit the growth of Bacillus cereus bacteria in the weak inhibition category.
References
Sabira S, et al..Nigella sativa monograph. Journal of Pharmacognosy and Phytochemistry, 103-106. Pakistan. 2015. Available from: https://www.researchgate.net/publication/317428423_Nigella_sativa_Monograph
Murali M, et al. Current knowledge on alleviating Helicobacter pylori infections through commonly known natural products: bench to beside. Inyegrative medicine research. (3), 111- 118.Malaysia. 2014. DOI : https://doi.org/10.1016/j.imr.2014.04.001
PMid:28664086 PMCid:PMC5481734
Hidayat L.N.R., Riyadi S.A., Gustiani S., Dwicahya A., 2022. Aplikasi ekstrak jintan hitam(Nigella sativa L.) sebagai zat antibakteri pada kain kapas dengan variasi metode., JurnalArenaTekstil, 37(1),pp. 9-18. DOI: https://doi.org/10.31266/at.v37i1.7730
Marlinda L., 2015. Effectivity of black cumin seeds extract to increase phagocytosis, Journal Majority,4(3),pp. 58-64
Agustianasari I.,Mulqie L., Choesrina R., 2016. Uji Aktivitas Antibakteri Ekstrak dan Fraksi Biji Jinten Hitam (Nigella sativaL.) terhadap Bakteri Bacillus subtilis dan Escherichia coli.Available from: https://karyailmiah.unisba.ac.id/index.php/farmasi/article/view/4195/0, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Bandung, 2(2), pp. 682-690
Novard M.F.A., Suharti N., RasyidR., 2019.Gambaran bakteri penyebab penyakit pada anakberdasarkan jenis spesimen dan pola resistensinyadi laboratorium RSUP Dr. M. Djamil Padang tahun 2014-2016, Jurnal Kesehatan Andalas,8(2),pp.26-32. DOI: https://doi.org/10.25077/jka.v8i2S.955
Sopandi T. and Wardah., 2014. Mikrobiologi Pangan Teori dan Praktik. Indonesia: Andi Publisher.Yogyakarta 494 hal
Arisanti R.R., Indriani C.,Wilopo S.A., 2018. Kontribusi agen dan faktor penyebab kejadian luar biasa keracunan pangan di Indonesia: kajian sistematis, BKM Journal of Community Medicineand Public Health, 334(3), pp. 99-106. DOI : https://doi.org/10.22146/bkm.33852
Patricia M. Tille, Bailey & Scott's Diagnostic Microbiology, 14 Ed, St. Louise Missouri, Elsevier, 2017.
Fera Astika, and Cita Hanif Muflihah, Usadha: Journal of Pharmacy, vol.2, No.1, 2023. ISSN 2827-9905. Available from : https://jar.llb.ums.ac.id/Index.php/ujp
Nevi Sulvita, Efektivitas minyak Habbatussauda (Nigella sativa L.) terhadap pertumbuhan Staphylococcus aureus, FK UMI,2018. DOI: https://doi.org/10.33096/umj.v3i2.40
Guntur Satrio P, Nurul Chusna, M.Priyadi, Uji Potensi Daya Hambat Ekstra Metanol biji jinten Hitam (Nigella sativa L.) terhadap bakteri Streptococcus, Jurnal Surya Medika (JSM), vol.6 No.1, Agustus 2020 Page 18-21. P-ISSN:2460-7266; e-ISSN 2655-2051 DOI: https://doi.org/10.33084/jsm.v6i1.1615
Mudzalifah. Efektifitas Efek Anti Mikroba Ekstrak Jintan Hitam terhadap Pertumbuhan Bakteri Salmonella Thypi. Fakultas Kedokteran Universitas Lampung. 2016;54–59.
Saleh FA, El-Darra N, Raafat K, El Ghazzawi I. Phytochemical Analysis of Nigella Sativa L. Utilizing GC-MS Exploring Its Antimicrobial Effects Against Multidrug-Resistant Bacteria. Pharmacogn J. 2018;10(1):99–105. DOI: https://doi.org/10.5530/pj.2018.1.18
Zuraida, Masdianto, Haniefa Zhuhruful Jannah; Anakes, Jurnal Analis Kesehatan, vol.8, no.1; Maret 2022, p-ISSN:2088-5687, eISSN:2745-6099 DOI: https://doi.org/10.37012/anakes.v8i1.872
Zeniusa P., Ramadhian M.R., Nasution S.H., Karima N., 2019.Uji Daya Hambat Ekstrak Etanol The Hijau Terhadap Escherichiacoli secara InVitro, Majority, 8(2), pp.136-143. Available from: http://digilib.unila.ac.id/29961/10/SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf
Lintang. Uji Efektivitas Ekstrak Jintan Hitam (Nigella sativa) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa. Fakultas Kedokt dan Ilmu Kesehatan UIN Syarif Hidayatullah. 2014; Available from: https://repository.uinjkt.ac.id/dspace/bitstream/123456789/25781/1/LINTANG%20SURYANING%20BHUMI.pdf
Anggita D, Yusriani Y, Abdi DA, Desiani V. Effectiveness of Chinese Leaf Extract and Leaf Plants (Jatropha Multifida L.) As Antibacterial to Bacterial Growth Staphylococcus Aureus In Vitro. Window of Health. 2018;1(1):29-33. DOI: https://doi.org/10.33368/woh.v0i0.13
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