Bay Leaf (Syzygium Polyanthum) Extract Effect on IL-10 and IL-6 Gene Expression in Traumatic Ulcer

Authors

  • Arina Shafia Universitas Islam Sultan Agung, Semarang, Indonesia
  • Setyo Trisnadi Universitas Islam Sultan Agung, Semarang, Indonesia
  • Agung Putra Universitas Islam Sultan Agung, Semarang, Indonesia

DOI:

https://doi.org/10.33533/jpm.v17i2.6914

Keywords:

Bay leaves, Traumatic ulcer, IL-6 levels, IL-10 levels

Abstract

The aims of this research is to prove the effect of bay leaf extract gel on IL-10 and IL-6 gene expression in traumatic ulcers. The research method using In vivo experimental study with a randomized posttest-only control group design. The total sample was 24 male Wistar rats, divided into four groups. Group K1 (given base gel), group K2 (given Kenalog in Orabase), group K3 (10% bay leaf extract gel), and group K4 (15% bay leaf extract gel) were given treatment for 5 days. IL-6 and IL-10 levels were tested using the One-Way Anova test, followed by the Tamhane Post Hoc test. This study revealed that there was a difference in IL-6 levels in the K4 group compared to the K1 group sig. (p = 0.007) and a significant difference in IL-10 levels in the K4 group compared to the K1 group. (p = 0.003) after being given topical treatment with bay leaf extract gel. The conclusion of this research is treatment with 15% bay leaf gel extract (Syzygium polyanthum) was proven to significantly increase IL-10 gene expression and decrease IL-6 gene expression in Wistar rats model of traumatic ulcers.

References

[1]. Langlais RP, Miller CS, Gehrig JS. Color Atlas of Common Oral Diseases. 5th Editio. Philadelphia: Lippincott Williams & Wilkins; 2016.

[2]. Amtha R, Marcia M, Aninda AI. Plester Sariawan Efektif Dalam Mempercepat Penyembuhan Stomatitis Aftosa Rekuren dan Ulkus Traumatikus. Maj Kedokt Gigi Indones. 2017;3(2):69.

[3]. Herawati E, Dwiarie TA. Temuan Klinis dan Manajemen Kasus Ulserasi Rongga Mulut Terkait Trauma Iatrogenik. J Kedokt Gigi Univ Padjadjaran. 2019;31(2).

[4]. Sunarjo L, Hendari R, Rimbyastuti H. Manfaat Xanthone Terhadap Kesembuhan Ulkus Rongga Mulut Dilihat dari Jumlah Sel PMN dan Fibroblast. ODONTO Dent J. 2015;2(2):14–21.

[5]. Johnson BZ, Stevenson AW, Prêle CM, Fear MW, Wood FM. The role of IL-6 in skin fibrosis and cutaneous wound healing. Biomedicines. 2020;8(5):1–18.

[6]. Rojas JM, Avia M, Martín V, Sevilla N. IL-10: A multifunctional cytokine in viral infections. J Immunol Res. 2017;2017.

[7]. Duque GA, Descoteaux A. Macrophage Cytokines: Involvement in Immunity and Infectious Diseases. Front Immunol. 2014;5(491):1–12.

[8]. Kresno SB. IMUNOLOGI: Diagnosis dan Prosedur Laboratorium. Edisi 5. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2013. 114 p.

[9]. Silalahi M. Syzygium polyanthum (Wight) Walp. (Botani, Metabolit Sekunder dan Pemanfaatan). J Din Pendidik. 2017;10(1):1–16.

[10]. Utama TIW. Formulasi Sediaan Krim Ekstrak Etanolik Daun Salam (Eugenia polyantha Wight.) Dengan Pengujian Aktivitasnya Sebagai Anti Inflamasi Topikal Pada Tikus. Universitas Sanata Dharma; 2014.

[11]. Lee W, Har I, Safinar I. Antioxidant activity, total phenolics and total flavonoids of Syzygium polyanthum (Wight) Walp leaves. Int J Med Arom Plants. 2012;vol 2(2):2249–4340.

[12]. Syarifah AL, Retnowati R, Soebiantoro S. Characterization of Secondary Metabolites Profile of Flavonoid from Salam Leaves (Eugenia polyantha) Using TLC and UVSpectrophotometry. Pharm Sci Res. 2019;6(3):155–63.

[13]. Tanjung YA, Refli Hasan, Rahmad Isnanta. Effect of Salam leaf extract (Syzygium Polyanthum) to serum IL-10 levels on Acute Coronary Syndrome in Wistar Strain Mice Model. J Endocrinol Trop Med Infect Dis. 2020;2(3):153–61.

[14]. Choy KW, Murugan D, Leong XF, Abas R, Alias A, Mustafa MR. Flavonoids as natural anti-inflammatory agents targeting nuclear factor-kappa B (NFκB) signaling in cardiovascular diseases: A mini review. Front Pharmacol. 2019;10(OCT):1–8.

[15]. Savira A, Mujayanto R, Amurwaningsih M. Bay Leaf (Syzygium Polyanthum) Extract Gel Effect on Tnf- Α Expression in Traumatic Ulcers Healing Process. ODONTO Dent J. 2020;7(1):25.

[16]. Shafia A, Mujayanto R, Feranisa A. Bay Leaf (Syzygium Polyanthum) Extract Effect on Il-10 Expression in Oral Ulcer. ODONTO Dent J. 2020;7(1):53.

[17]. Rosada A, Mujayanto R, Poetri AR. Ekstrak Daun Salam Dalam Meningkatkan Ekspresi Fibroblast Growth Factor Pada Ulkus Traumatik Rongga Mulut. ODONTO Dent J. 2020;7(2):90.

[18]. Ernawati DS, Puspa A. Expression of vascular endothelial growth factor and matrix metalloproteinase-9 in Apis mellifera Lawang propolis extract gel-treated traumatic ulcers in diabetic rats. Vet World. 2018;11(3):304–9.

[19]. Surboyo MDC, Mahdani FY, Ernawati DS, Hadi P, Hendarti HT, Parmadiati AE, et al. Number of macrophages and transforming growth factor β expression in citrus limon L. Tlekung peel oil-treated traumatic ulcers in diabetic rats. Trop J Pharm Res. 2019;18(7):1427–33.

[20]. Damaiyanti DW, Soesilowati P, Arundina I, Sari RP. Effectiveness of gold sea cucumber (Stichopus hermanii) extracts in accelerating the healing process of oral traumatic ulcer in rats. Padjadjaran J Dent. 2019;31(3):208.

[21]. Fikri MA. Uji Antiinlamasi Ekstrak Etanol Daun Salam (Syzygium polyanthum) dan Daun Kemangi (Ocimum sanctum) pada Tikus Hiperurisemia. Universitas Muhammadiyah Surakarta; 2018.

[22]. Hämäläinen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E. Anti-inflammatory effects of flavonoids: Genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-κB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-κB activation along with their inhibitory effect on i. Mediators Inflamm. 2007;2007.

[23]. Rahmani AH, Alsahli MA, Khan AA, Almatroodi SA. Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats. Metabolites. 2023;13(1):130.

[24]. Waslyk A, Bakovic M. Biological Activity and Therapeutic Potential of Quercetin for Inflammatory Bowel Disease. J Food Sci Nutr Res. 2021;04(02):94–117.

[25]. Tang J, Diao P, Shu X, Li L, Xiong L. Quercetin and Quercitrin Attenuates the Inflammatory Response and Oxidative Stress in LPS-Induced RAW264.7 Cells: In Vitro Assessment and a Theoretical Model. Biomed Res Int. 2019;2019.

[26]. Kant V, Jangir BL, Kumar V, Nigam A, Sharma V. Quercetin accelerated cutaneous wound healing in rats by modulation of different cytokines and growth factors. Growth Factors. 2020;38(2):105–19.

[27]. Zhang F, Feng J, Zhang J, Kang X, Qian D. Quercetin modulates AMPK/SIRT1/NF‑κB signaling to inhibit inflammatory/oxidative stress responses in diabetic high fat diet‑induced atherosclerosis in the rat carotid artery. Exp Ther Med. 2020;20(6):1–1.

[28]. Saeedi-Boroujeni A, Mahmoudian-Sani MR. Anti-inflammatory potential of Quercetin in COVID-19 treatment. J Inflamm (United Kingdom). 2021;18(1):1–9.

[29]. Aravinthan A, Kim JH, Antonisamy P, Kang CW, Choi J, Kim NS, et al. Ginseng total saponin attenuates myocardial injury via anti-oxidative and anti-inflammatory properties. J Ginseng Res. 2015;39(3):206–12.

[30]. Zhang XX, Ito Y, Liang JR, Liu JL, He J, Sun WJ. Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund’s complete adjuvant induced arthritis in rats. Int Immunopharmacol. 2014;23(2):407–16.

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Published

2023-12-15

How to Cite

Shafia, A., Trisnadi, S., & Putra, A. (2023). Bay Leaf (Syzygium Polyanthum) Extract Effect on IL-10 and IL-6 Gene Expression in Traumatic Ulcer. Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 17(2), 204–213. https://doi.org/10.33533/jpm.v17i2.6914