The Generation Time of Escherichia Coli Bacteria Based On Variaton In Eosin Methylene Blue Agar Media PH

Diah Lestari; Indah Prasetyowati; Mega Mirawati

doi:10.33533/jpm.v17i2.6405

Authors

Diah Lestari Poltekkes Kemenkes Jakarta III
Indah Prasetyowati Poltekkes Kemenkes Jakarta III
Mega Mirawati Poltekkes Kemenkes Jakarta III

DOI:

https://doi.org/10.33533/jpm.v17i2.6405

Keywords:

Eosin methylene blue agar media, Escherichia coli, pH variation

Abstract

Bacteria require optimum environmental conditions to support cell growth and reproduction. States that are less than optimal cause bacteria to adapt and can infect humans for invasion of other organs. The speed of bacteria to infect is influenced by the rate and time of generation of bacteria in multiplying cells. This study aims to determine the effect of variations in pH (4,8; 5,8; 6,8; 7,8; and 8,8) in Eosin Methylene Blue Agar (EMBA) media on the generation time of Escherichia coli bacteria. Researchers used the True Experiment Post Only Control Design and One Way ANOVA test. The results showed that at a variation of pH 4,8 there was no growth; pH 5,8 with a generation time of 1,5-24,9; pH 6,8 with a generation time of 1,5-21,7 minutes; pH 7,8 with a generation time of 1,6-22,3 minutes; and pH 8,8 with a generation time of 1,6-22,8 minutes. The study concludes is that there is a significant effect of the variation in pH of the EMBA media on the generation time of Escherichia coli bacteria with a p-value of 0,000 < α 0,05, with the fastest generation time at pH 6,8.

Author Biographies

Diah Lestari, Poltekkes Kemenkes Jakarta III

Teknologi Laboratorium Medik Politeknik Kesehatan Jakarta III

Indah Prasetyowati, Poltekkes Kemenkes Jakarta III

Medical Laboratory Technology

Mega Mirawati, Poltekkes Kemenkes Jakarta III

Medical Laboratory Technology

References

[1]. Yang X, Wang H. Pathogenic E. coli. Lacombe Res Centre, Lacombe Canada. 2014

[2]. Rahayu WP, Nurjanah S, Komalasari E. Escherichia coli: Patogenitas,Analisis, dan Kajian Risiko. Vol. 53, Journal of Chemical Information and Modeling. 2018. 5 p.

[3] CDC. Escherichia coli (E. coli) [Internet]. Centers for Disease Control and Prevention. 2014 [cited 2022 Sep 18]. Available from: http://www.cdc.gov/ecoli/general/ind%0Aex.htm

[4]. Tumagor YL. Gambaran Bakteri Escherichia coli pada Cincau Systematic Review. Politeknik Kesehatan Kemenkes Medan; 2021.

[5]. Abna IM. Pertumbuhan dan Perkembangan Mikroba Pertemuan III-IV. Jakarta; 2020.

[6]. Madigan michael T, Martinko JM, Bender KS, Buckley DH, Stahl DA. Biologi Mikroorganisme. 14th ed. Jayadi CY, Nirwanto mohammad R, editors. Jakarta: EGC; 2016. 1–214 p.

[7]. Arivo D, Annissatussholeh N. Pengaruh Tekanan Osmotik pH, dan Suhu Terhadap Pertumbuhan Bakteri Escherichia Coli. J Ilmu Kedokt dan Kesehat. 2017;4(3):153–60.

[8]. Merck. Merck Microbiology Manual 12th Edition. 12th ed. Merck. Darmstadt, Germany; 2016. 689 p.

[9]. Arianda D. Buku Saku Bakteriologi. AM-publishing T, editor. AM-publishing; 2016. 100 p.

[10]. Setiawati MR, Suryatmana P, Herdiyantoro D, Ilmiyati Z. Karakteristik Pertumbuhan dan Waktu Generasi Isolat Azotobacter sp. dan Bakteri Endofitik Asal Ekosistem Lahan Sawah. JurAgroekotek 6. 2014;(1):12–20.

[11]. Pratiwi K. Modul MK. Mikrobiologi Pangan. Bali; 2017.

[12]. Sariadji K, Wati M, Syamsidar, A N, Sundari, Khariri, et al. Waktu Regenerasi Bakteri Vibrio cholerae Pada Medium APW. 2015;151(1):10–7.

[13]. Hikmah J. Pengaruh pH dan Suhu Terhadap Aktivitas Antibakteri Bekatul Terfermentasi Oleh Rhizopus oryzae [Internet]. Vol. 63. Universitas Islam Negeri Maulana Malik Ibrahim Malang; 2018. Available from: http://forschungsunion.de/pdf/industrie_4_0_umsetzungsempfehlungen.pdf%0Ahttps://www.dfki.de/fileadmin/user_upload/import/9744_171012-KI-Gipfelpapier-online.pdf%0Ahttps://www.bitkom.org/ sites/default/files/ pdf/Presse/Anhaenge-an-PIs/ 2018/180607 -Bitkom

[14]. Yusuf M, Kurniawan H, Pahlevi ABR, Anton, Budiman C, Arief II. Peranan Pompa Proton pada Pertumbuhan Escherichia coli di Lingkungan pH Alkali. Jurnail Sain Peternak Indones. 2020;15(1):83–90.

[15]. Kadri AN, Gelgel KTP, Suarjana IGK. Perbedaan Cara Penyebaran Suspensi terhadap Jumlah Bakteri pada Media Eosin Methylene Blue Agar. Indones Med Veterinus. 2015;

[16]. Azhari F, Winarsa R, Siswanto, Muzakhar K, Utarti E, Sutoyo, et al. Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.). Berk Saintek. 2021;2(9):86–94.

[17]. Suriani S, Soemarno, Suharjono. Pengaruh Suhu dan pH terhadap Laju pertumbuhan Lima Isolat Bakteri Anggota Genus Pseudomonas yang diisolasi dari Ekosistem Sungai Tercemar Deterjen di sekitar Kampus Universitas Brawijaya. J-Pal. 2013;3(2):58–62.

[18]. Ulfa M. Patogenesis Escherichia coli. UMY Magister Adm Rumah Sakit [Internet]. 2018; Available from: https://mars.umy.ac.id/patogenesis-escherichia-coli/