The Relationship Between Cervical Cancer Severity and miRNA-205 Expression in Bali

Kartika Sari; Desak Putu Oki Lestari; Novitasari Novitasari; Yudhi Nugraha; Vivitri Dewi Prasasty; Septi Panca Sakti

doi:10.33533/jpm.v16i2.4106

Authors

Kartika Sari Faculty of Medicine and Health Sciences, Warmadewa University Bali
Desak Putu Oki Lestari Faculty of Medicine and Health Sciences, Warmadewa University Bali
Novitasari Novitasari The National Research and Inovation Agency, Republic of Indonesia http://orcid.org/0000-0001-8340-2694
Yudhi Nugraha Spanish National Cancer Institute (CNIO) Madrid http://orcid.org/0000-0003-1186-4093
Vivitri Dewi Prasasty School of Basic Pharmaceutical and Toxicological Sciences, University of Monroe
Septi Panca Sakti International Health Sciences, Fulda University of Applied Sciences

DOI:

https://doi.org/10.33533/jpm.v16i2.4106

Keywords:

Cervical cancer, Biomarker, HPV, miRNA-205

Abstract

Cervical cancer is the one of leading cause of death for women worldwide. It occurs due to infection with the human papilloma virus (HPV) which attacks the cervix in the female reproductive organs. The detecting cervical cancer method is by using pap smears and liquid based cytology (LBC) and vaccinations. However, both methods are limited in detecting cervical cancer quickly and accurately. This study aims to find potential biomarkers that will be used to quickly detect the development of cervical cancer. Large-scale studies have shown that microRNA-205 (miRNA) regulation has a very important role in various types of cancer including cervical cancer. The extraction and synthesis of cDNA from 26 preparations of cervical cancer paraffin blocks were measured by quantitative real time PCR to evaluate the expression of miRNA-205 as a biomarker of cervical cancer in cervical cancer samples. Statistical results showed that there was a significant difference in the expression of miRNA-205 which was relatively higher in cervical cancer patients (p<0.05) miRNA-205. In addition, the increased expression of miRNA-205 is associated with an increase in the severity of cervical cancer. We conclude that miRNA-205 is a potential candidate for cervical cancer molecular biomarker.

Author Biographies

Kartika Sari, Faculty of Medicine and Health Sciences, Warmadewa University Bali

Physiology and Biochemistry Department

Desak Putu Oki Lestari, Faculty of Medicine and Health Sciences, Warmadewa University Bali

Physiology and Biochemistry Department

Novitasari Novitasari, The National Research and Inovation Agency, Republic of Indonesia

Research Centre for Testing Technology

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