Effect of Ethanol Extract Karamunting Leaf (Rhodomyrtus Tomentosa (Aiton) Hassk) on Testosterone Hormone Levels of Diabetes Mellitus Model Rats

Authors

  • Husnul Khatimah Departement of Biomedic, Faculty of Medicine, Lambung Mangkurat University, Jl Veteran no 128, Banjarmasin
  • Lena Rosida Departement biomedic, Faculty of Medicine, Lambung Mangkurat University, Jl Veteran no 128, Banjarmasin
  • Siti Kaidah Departement biomedic, Faculty of Medicine, Lambung Mangkurat University, Jl Veteran no 128, Banjarmasin
  • Ida Yuliana Departement biomedic, Wetland Disease Center, Faculty of Medicine, Lambung Mangkurat University, Jl Veteran no 128, Banjarmasin

DOI:

https://doi.org/10.33533/jpm.v15i2.3452

Keywords:

Diabetes mellitus, Karamunting Leaf, Testosterone

Abstract

Karamunting leaf is a natural ingredient in South Kalimantan and is used for the treatment of diabetes mellitus. Diabetes mellitus (DM) can decrease hormone testosterone. Research on Karamunting leaves on testosterone levels had not been widely studied. The aim was to analyse the effect of administration ethanol extract Karamunting leaf on testosterone levels in diabetic rats induced by streptozotocin. The method was true experimental,  Posttest Control Group. The research groups were divided into 4 groups. The control group giving streptozotocin induction at dosage 40 mg/kg bw rats intra-peritoneally. The treatment group was a group of rats DM  treated with ethanol extract of Karamunting leaf at dosage 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg bw. The treatment in the research group was carried out for 14 days. On day 15, blood was taken to measure testosterone levels by the ELISA method and glucose blood. The data were analysed with Kolmogorov Smirnov and Mann Whitney. Testosterone levels increased with administration of ethanol extract Karamunting leaves at dose 100 mg/kg bw (16.58 ± 2.8) and 400 mg/kg bw (16.17 ± 2.07) when compared with the control group (14.56 ± 0.8). Administration of ethanolic extract of Karamunting leaf in DM rats has an effect on testosterone levels. 

References

Sutomo, Arnida, Hernawati F., dan Yuwono M. Kajian farmakognostik simplisia daun karamunting (Rhodomyrtus tomentosa Ait. Hassk) asal pelaihari salimantan selatan. Sains dan Terap Kim. 2010;4(1):41–2.

Sari LORK. Pemanfaatan obat tradisional dengan Pertimbangan Manfaat dan Keamanannya. Maj Ilmu Kefarmasian. 2006;3(1):1–17.

Dewi I. Uji Aktivitas Antidiabetes Ekstrak Etanol Daun Salam (Eugenia Polyantha) terhadap Tikus Galur Wistar yang Diinduksi Aloksan. Surakarta; 2013.

Sulistyo, N.H., Hernawaty, F., Shafwatunnida, L., Rusida E.R., Rahman M. Uji Aktivitas Daun Karamunting (Rhodomyrtus tomentosa) Sebagai Obat Diabetes Melitus Di Daerah Pelaihari Kecamatan Pelaihari Kabupaten Tanah Laut Kalimantan Selatan. 2007.

Sinata, N dan Arifin H. Antidiabetes dari fraksi air daun karamunting (Rhodomyrtus tomentosa (Ait.) Hassk.) terhadap kadar glukosa darah mencit diabetes. J Sains Farm Klin. 2016;3(1):72–8.

Tandi J. Pengaruh ekstrak etanol daun jambu air (Syzygium aqueum (Burm f.) Alston) terhadap glukosa darah, dan kreatinin tikus putih (Rattus norvegicus). J Trop Pharm Chem. 2017;4(2):45–50.

American Diabetes Association (ADA). Medical advice for people with diabetes in emergency situations. America: American Diabetes Association Journal; 2012.

Federation ID. IDF diabetes atlas update poster. Brussel, Belgium: International Diabetes Federation; 2014.

Dinas Kesehatan Provinsi Kalimantan Selatan. Jumlah penderita Penyakit diabetes mellitus.

Ram Niwas Jangir and Gyan Chand Jain. Diabetes mellitus induced impairment of male reproductive functions: a review. Curr Diabetes Rev. 2014;10:147–57.

Zhao H, Xu S, Wang Z et al. Repetitive exposures to low-dose Xrays attenuate testicular apoptotic cell death in streptozotocin induced diabetes rats. Toxicol Lett. 2010;192:356–64.

Mohasseb M, Ebied S, Yehia MAH HN. Testicular oxidative damage and role of combined antioxidant supplementation in experimental diabetic rats. J Physiol Biochem. 2011;67:185–94.

Pontes DA, Fernandes GS, Piffer RC, Gerardin DC, Pereira OC KW. Ejaculatory dysfunction in streptozotocin induced diabetic rats: the role of testosterone. Pharmacol Rep. 2011;63:130–8.

Vikram A, Kushwaha S JG. Relative influence of testosterone and insulin in the regulation of prostatic cell proliferation and growth. Steroids. 2011;76:416–23.

Febriyanto G, Saleh MI, Theodorus T. Efektivitas Antidiabetes Fraksi Air Daun Karamunting (Rhodomyrtus Tomentosa (Ait.) Hassk.) terhadap Kadar Glukosa Darah dan Sekresi Insulin pada Tikus Model Diabetes. J Ilm Kedokt Wijaya Kusuma. 2021;10(1):57.

Pertiwi L. Aktivitas antidiabetes ekstrak dan fraksi daun karamunting (Rhodomyrtus tomentosa (ait.) hassk.) pada tikus putih jantan galur Wistar terinduksi streptozotosin (Skripsi). Palembang: Universitas Sriwijaya; 2020. Palembang; 2020.

Saputra NT, Suartha IN, Dharmayudha AAGO. Agen Diabetagonik Streptozotocin untuk Membuat Tikus Putih Jantan Diabetes Mellitus. Bul Vet Udayana. 2018;10(2):116.

Hayati F, Widyarini S, Hakim L, Ngatidjan M. Pengaruh pemberian ekstrak akar pasak bumi terstandar terhadap gambaran histopatologik testis dan konsentrasi testis pada tikus. J Ilmu kefarmasian Indones. 2012;10(1):50–6.

Valentovic, M. A., Alejandro, N., Betts Carpenter, A., Brown, P. I., & Ramos K. Streptozotocin (STZ) diabetes enhances benzo (alpha) pyrene induced renal injury in Sprague Dawley rats. Toxicol Lett. 2006;164(3):214–220.

Eleazu, C. O., Eleazu, K. C., Chukwuma, S., & Essien UN. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. J Diabetes Metab Disord. 2013;12(1):60.

Dimakopoulou A, Jayasena CN, Radial UK et al. Animal model of diabetes-related male hypogonadism. Front Endocrinol (Lausanne). 2019;10(628):1–4.

Kerstin Nowotny, Tobias Jung, Annika Höhn DW and TG. Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. Biomolecules. 2015;5:194–222.

Yun-Tao Zhao, Ya-Wei Qi, Chuan-Yin Hu S-HC and YL. advanced glycation end products inhibit testosterone secretion by rat leydig cells by inducing oxidative stress and endoplasmic reticulum stress. Int J Mol Med. 2016;38:659–65.

Rho HW, Lee JN, Kim HR, Park BH, Park JW. Protective mechanism of glucose against alloxan-induced β-cell damage: Pivotal role of ATP. Exp Mol Med. 2000;32(1):12–7.

Dina MS, Dasrul, Sugito, S Wahyuni TAT& I. Penurunan jumlah sel leydig dan sel sertoli Tikus Putih (Rattus norvegicus) Strain Wistar setelah pemberian formalin. JMVET. 2017;1(2):203–9.

Malini DM, Madihah, DA Khoirunnisa, I Sasmita, N Ratningsih KA& WH. Ekstrak etanol kulit buah jengkol menurunkan kadar glukosa dan meningkatkan hormon insulin Tikus diabetes yang diinduksi Streptozotocin. Veteriner. 2019;20(36):65–73.

Arjadi, Fitranto dan Susatyo P. Regenerasi Sel Pulau Langerhans Pada Tikus Putih (Rattus norvegicus) Diabetes yang Diberi Rebusan Daging Mahkota Dewa (Phaleria macrocarp (scheff.) Boerl.). J Med Fac Jendral Soedirman Univ. 2010;2(2):117–26.

Heim KE AT& DB. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationship. J Nutr Biochem. 2002;13:572–84.

Liahaq M, Amanat., Sudrajat. S. Uji efektivitas batang karamunting (Melastoma malabathricum L) terhadap penurunan kadar glukosa darah mencit (Mus musculus) diabetes. Sci East Borneo. 2015;3(3).

Isdamayani L dan Panunggal B. Kandungan flavonoid, total fenol, dan antioksidan snack bar sorgum sebagai alternatif makanan selingan penderita diabetes mellitus tipe 2. J Nutr Coll. 2015;4(2):342–9.

Kumari M & Jain S. Tannins: An Antinutrient with Positive Effect to Manage Diabetes. Res J Recent Sci. 2012;1(12):70–3.

Ballester J, Dominguez J, Muñoz MC, Sensat M, Rigau T, Guinovart JJ & Rodriguez-Gil JE. Tungstate treatment improves Leydig cell function in streptozotocin-diabetic rats. J Androl.

;26(6):706–15.

Sabirosi BG TP& WD. Ekspressi Tumor Necrossis Factor-Alpha (TNF-α) pada Tikus (Rattus norvegicus) model diabetes mellitus tipe 1 hasil induksi streptozotocin yang diterapi dengan ekstrak etanol rimpang Kunyit (Curcuma longa L.). J Kedokt Hewan. 2014;4(3):1–9.

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Published

2021-12-20

How to Cite

Khatimah, H., Rosida, L., Kaidah, S., & Yuliana, I. (2021). Effect of Ethanol Extract Karamunting Leaf (Rhodomyrtus Tomentosa (Aiton) Hassk) on Testosterone Hormone Levels of Diabetes Mellitus Model Rats. Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 15(2). https://doi.org/10.33533/jpm.v15i2.3452