UJI TOKSIK SUSPENSI SEL PUNCA CD34+ MANUSIA PADA TIKUS SPRAGUE DAWLEY (SD)

Authors

  • Basuki Supartono FK UPN "Veteran" Jakarta

DOI:

https://doi.org/10.33533/jpm.v8i2.679

Keywords:

toxicity test, stem cell, CD34 cell, scaffold, growth factors, naive rats, tissue engineering

Abstract

Introduction :  Research of CD34+ cells intervention to rats is not yet done. This researchis the latest research which investigated the toxic reaction of CD34+ hematopoietic stem cell intervention in non engineered rats.

Methods : 3 Sprague Dawley rats, male, 7 months, 285+18,5 g. Rat 1 : received intraartricular injection of human peripheral blood CD34+ cells (105 cells). Rat 2 : received intraartricular injection of human cord blood CD34+ cells (105 cells). Rat 3 : received intraartricular injection of human cord blood CD34+ cells (106 cells), hyaluronic acid and grown factors. All rats were examined hematological, blood chemistry parameters and microscopic of the liver and kidney.

Result : All rats showed good condition. There is slight increasing parameter in hematological and blood chemistry but liver and kidney in normal microscopic condition. No hiperacute rejection reaction : edema, systemic bleeding. There is no hyper acute and acute reaction.

Conclusion :  Human CD34+ cells suspension with or without scaffold and growth factors does not cause toxicity reaction in naïve Sprague Dawley rats.

 

Author Biography

Basuki Supartono, FK UPN "Veteran" Jakarta

Laboratorium Stem Cell

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Published

2019-04-04

How to Cite

Supartono, B. (2019). UJI TOKSIK SUSPENSI SEL PUNCA CD34+ MANUSIA PADA TIKUS SPRAGUE DAWLEY (SD). Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 8(2). https://doi.org/10.33533/jpm.v8i2.679