Basuki Supartono


Introduction :  Research of CD34+ cells intervention to rats is not yet done. This researchis the latest research which investigated the toxic reaction of CD34+ hematopoietic stem cell intervention in non engineered rats.

Methods : 3 Sprague Dawley rats, male, 7 months, 285+18,5 g. Rat 1 : received intraartricular injection of human peripheral blood CD34+ cells (105 cells). Rat 2 : received intraartricular injection of human cord blood CD34+ cells (105 cells). Rat 3 : received intraartricular injection of human cord blood CD34+ cells (106 cells), hyaluronic acid and grown factors. All rats were examined hematological, blood chemistry parameters and microscopic of the liver and kidney.

Result : All rats showed good condition. There is slight increasing parameter in hematological and blood chemistry but liver and kidney in normal microscopic condition. No hiperacute rejection reaction : edema, systemic bleeding. There is no hyper acute and acute reaction.

Conclusion :  Human CD34+ cells suspension with or without scaffold and growth factors does not cause toxicity reaction in naïve Sprague Dawley rats.


Kata Kunci

toxicity test; stem cell; CD34 cell; scaffold; growth factors; naive rats; tissue engineering

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DOI: http://dx.doi.org/10.33533/jpm.v8i2.679


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